RnoEX6034026 @ rn6
Exon Skipping
Gene
ENSRNOG00000049033 | Racgap1
Description
Rac GTPase-activating protein 1 [Source:RGD Symbol;Acc:1305912]
Coordinates
chr7:141289514-141292899:-
Coord C1 exon
chr7:141292830-141292899
Coord A exon
chr7:141290976-141291026
Coord C2 exon
chr7:141289514-141289594
Length
51 bp
Sequences
Splice sites
3' ss Seq
CATGATCTAATTGCCAATAGGAC
3' ss Score
6.68
5' ss Seq
AGGGTATGT
5' ss Score
8.34
Exon sequences
Seq C1 exon
ACTGTCAACGATTGATGAATCTGGCTCCATTTTATCAGATATCAGCTTTGACAAGACTGATGAATCACTG
Seq A exon
GACTGGGATTCTTCTTTGAAGAATTTCAAAATGAAGAAACGAGAAAAGAGG
Seq C2 exon
CGCTCCAGCAGTAGACAGTTCATCGATGGCCCTCCTGGGCCTGTGAAGAAAACGTGTTCCATTGGCTCTACAATAGACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000049033-'14-11,'14-10,16-11=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.139 A=0.471 C2=0.815
Domain overlap (PFAM):
C1:
PF059116=DUF869=PD(5.0=29.2)
A:
NO
C2:
NO
Main Skipping Isoform:
ENSRNOT00000071885fB12043
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGATTGATGAATCTGGCTCCA
R:
GGTCTATTGTAGAGCCAATGGAACA
Band lengths:
142-193
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
PMID: 19468302
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, fluorescence technology, mutation analysis, mutation disrupting interaction, protein kinase assay, western blot. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]