MmuEX1021974 @ mm10
Exon Skipping
Gene
ENSMUSG00000023015 | Racgap1
Description
Rac GTPase-activating protein 1 [Source:MGI Symbol;Acc:MGI:1349423]
Coordinates
chr15:99632715-99642971:-
Coord C1 exon
chr15:99642883-99642971
Coord A exon
chr15:99634242-99634295
Coord C2 exon
chr15:99632715-99632795
Length
54 bp
Sequences
Splice sites
3' ss Seq
CATAGTCTAATTGCCAACAGGAC
3' ss Score
5.89
5' ss Seq
AGGGTATGT
5' ss Score
8.34
Exon sequences
Seq C1 exon
AAAGATGGATACTACAATGGTGAATTTGTGGACTCTGTTTGAGCAGCTTGTGCGCCGGATGGAGATTATCAATGAAGGAAATGAAAGCA
Seq A exon
GACTGGGATTCTTCTTTGGTGAAGAATTTCAAAATGAAGAAACGAGAGAAGAGG
Seq C2 exon
CGCTCCAACAGTAGACAGTTCATCGACGGCCCTCCTGGGCCTGTGAAGAAAACTTGTTCCATTGGCTCTACAGTAGACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000023015_MULTIEX1-4/7=C1-5
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.023 A=0.326 C2=0.815
Domain overlap (PFAM):
C1:
PF059116=DUF869=PU(15.3=62.1)
A:
NO
C2:
NO
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTGGACTCTGTTTGAGCAGC
R:
GGTCTACTGTAGAGCCAATGGA
Band lengths:
142-196
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
PMID: 19468302
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, fluorescence technology, mutation analysis, mutation disrupting interaction, protein kinase assay, western blot. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types