BtaEX0031460 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000015553 | SCAI
Description
suppressor of cancer cell invasion [Source:HGNC Symbol;Acc:HGNC:26709]
Coordinates
chr11:95938364-95941602:-
Coord C1 exon
chr11:95941530-95941602
Coord A exon
chr11:95940576-95940719
Coord C2 exon
chr11:95938364-95938537
Length
144 bp
Sequences
Splice sites
3' ss Seq
ATCTTCCTAACCGTGAACAGTGC
3' ss Score
5.3
5' ss Seq
AAGGTAAGT
5' ss Score
11
Exon sequences
Seq C1 exon
AATTTCACAAACTTATTTGGACAGCCACTAGTCTGCTTGCTTTCTCCTACAGCCTACCCAAAAGCTTTACAAG
Seq A exon
TGCTGGTGTCTCTCTGTGGGGGATGGCATTTTACTGCTACTTCCCAGCCAGAAGTCCTGTCTGCTGATATAGTTTGACACAGGAGGTGATGGTCAGCATTTGAATGCCCGAGAGAAGCTGGAGACTTTTCACCGTTGTTTTAAG
Seq C2 exon
ATCAATCTCAACGAGGTAGCCTGTTCACTCTGTTTTTGAACAATCCTCTAATGGCCTTCCTATTTGTCTCTGGACTGTCAAGCATGCGTAGAGGCCTATGGGAAAAGTGTCAAGAATATCTTCGAAAAATCAACCGTGACATTGCCCAGCTACTGACCCATTCACGTTCAATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000015553_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF120703=DUF3550=FE(4.8=100)
A:
PF120703=DUF3550=PD(3.1=50.0)
C2:
PF120703=DUF3550=FE(11.7=100)
Main Inclusion Isoform:
ENSBTAT00000020665fB6145
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAACTTATTTGGACAGCCACT
R:
AACGTGAATGGGTCAGTAGCTG
Band lengths:
234-378
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.550, FDR=0.000), PC9 No Change at 14 days (FC=1.445, FDR=0.134), Late Xenograft No change (FC=0.990, FDR=0.084).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development