MmuEX0041122 @ mm10
Exon Skipping
Gene
ENSMUSG00000035236 | Scai
Description
suppressor of cancer cell invasion [Source:MGI Symbol;Acc:MGI:2443716]
Coordinates
chr2:39080374-39083610:-
Coord C1 exon
chr2:39083538-39083610
Coord A exon
chr2:39082710-39082927
Coord C2 exon
chr2:39080374-39080547
Length
218 bp
Sequences
Splice sites
3' ss Seq
AACTCCCAGTCATTTCCTAGGTC
3' ss Score
3.9
5' ss Seq
AAGGTAAGT
5' ss Score
11
Exon sequences
Seq C1 exon
AATTTTACAAATTTGTTTGGACAGCCACTCGTCTGCCTTCTTTCTCCCACGGCATATCCAAAAGCTTTGCAAG
Seq A exon
GTCAAATGTTAAGTGAAACTTCACACTGTCTTAGCAGTACTTGAACTGTTTCACTGTCTCCCTGACTGTGAATAGTGCCGATGTCTCTCTGTGGGAGATGGCATTTCACTGCTACTTCCCAGCCCGAAGTCCTGTCTGCTGACATAGTTTGACACAGGAAGTGATGGTCAGCACTTGAATGCCTGAGAGAAGCTGGAGACTTACACCGTTGTTTTAAG
Seq C2 exon
ATCAGTCTCAGCGAGGTAGCCTCTTCACGCTCTTTTTGAACAATCCTCTAATGGCCTTCCTATTTGTGTCTGGATTGTCAAGCATGCGTAGAGGCCTGTGGGAAAAGTGTCAAGAATATCTTCGAAAAATCAACCGTGATATTGCCCAGCTACTGACCCATTCACGTTCAATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035236_MULTIEX2-1/2=C1-2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
Show structural model
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF120703=DUF3550=FE(32.0=100)
A:
PF120703=DUF3550=PD(14.7=42.3)
C2:
PF120703=DUF3550=FE(11.7=100)
Main Inclusion Isoform:
ENSMUST00000204093fB3583
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTACAAATTTGTTTGGACAGCCACT
R:
CTATTGAACGTGAATGGGTCAGT
Band lengths:
244-462
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.550, FDR=0.000), PC9 No Change at 14 days (FC=1.445, FDR=0.134), Late Xenograft No change (FC=0.990, FDR=0.084).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types