BtaEX0031864 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000012228 | SEMA4A
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:HGNC Symbol;Acc:HGNC:10729]
Coordinates
chr3:14660948-14671896:-
Coord C1 exon
chr3:14671746-14671896
Coord A exon
chr3:14663382-14663500
Coord C2 exon
chr3:14660948-14661048
Length
119 bp
Sequences
Splice sites
3' ss Seq
TCAACTCTCTCTCTCCACAGGCA
3' ss Score
13.22
5' ss Seq
CAGGTGGGA
5' ss Score
6.71
Exon sequences
Seq C1 exon
GCACGTTGGTGGGACCAGGAGCTCTGCTGTCTGTGCTTTCTCACTCAAGGACATCAAGAGTGTCTTTGAGGGGAAGTATAAGGAATTGGACAAAGAGACTTCACGCTGGACTACTTATGAATATCCTGATATCAGTCCACGGCCAGGCAGT
Seq A exon
GCACAGGCTCCCTCCACAAGGCTGTGGTGAGTGGGGACCACAGTGCTTATCTGGTGGAGGAGATTCAGCTGTTCCCTGACCCTGAACCTGTTCGAAACCTGCAGCTGGCCCCTACCCAG
Seq C2 exon
GAAGTCCTGGAAACAGGACATGCAGCAAGGGAACCCAGAGTGGGCATGTGCCAGTGGCCCCATGGGCAGGAGTCTGGGGCCCAGGAGCCGCCCCCAAATCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012228_MULTIEX1-2/3=C1-C2
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.059 A=0.025 C2=0.029
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(12.0=100)
A:
PF0140314=Sema=FE(9.4=100)
C2:
PF0143720=PSI=PD(32.1=48.6)
Main Skipping Isoform:
ENSBTAT00000016223fB6229
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGGGACCAGGAGCTCTG
R:
GATTTGGGGGCGGCTCCT
Band lengths:
244-363
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development