MmuEX6071975 @ mm10
Exon Skipping
Gene
ENSMUSG00000028064 | Sema4a
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:MGI Symbol;Acc:MGI:107560]
Coordinates
chr3:88438183-88440878:-
Coord C1 exon
chr3:88440698-88440878
Coord A exon
chr3:88438443-88438561
Coord C2 exon
chr3:88438183-88438340
Length
119 bp
Sequences
Splice sites
3' ss Seq
CCTTGTCTCTGTCTCTGCAGCCA
3' ss Score
10.77
5' ss Seq
CAGGTGAGA
5' ss Score
9.22
Exon sequences
Seq C1 exon
TGCTCCATGGGCCCCTCCTCTGACAAAGCCTTGACCTTCATGAAGGACCATTTTCTGATGGATGAGCACGTGGTAGGAACACCCCTGCTGGTGAAGTCTGGTGTGGAGTACACACGGCTTGCTGTGGAGTCAGCTCGGGGCCTTGATGGGAGCAGCCATGTGGTCATGTATCTGGGTACCT
Seq A exon
CCACGGGGTCCCTGCACAAGGCTGTGGTGCCTCAGGACAGCAGTGCTTATCTCGTGGAGGAGATTCAGCTGAGCCCTGACTCTGAGCCTGTTCGAAACCTGCAGCTGGCCCCCGCCCAG
Seq C2 exon
GGTGCAGTGTTTGCAGGCTTCTCTGGAGGCATCTGGAGAGTTCCCAGGGCCAATTGCAGTGTCTACGAGAGCTGTGTGGACTGTGTGCTTGCCAGGGACCCTCACTGTGCCTGGGACCCTGAATCAAGACTCTGCAGCCTTCTGTCTGGCTCTACCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028064-'41-48,'41-47,44-48=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.049 A=0.100 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(14.5=100)
A:
PF0140314=Sema=FE(12.0=100)
C2:
PF0143720=PSI=PU(61.4=66.0)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCTGGTGAAGTCTGGTGTG
R:
AGACAGAAGGCTGCAGAGTCT
Band lengths:
244-363
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types