BtaEX0037539 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000001697 | TRA2B
Description
Bos taurus transformer 2 beta homolog (Drosophila) (TRA2B), mRNA. [Source:RefSeq mRNA;Acc:NM_001034776]
Coordinates
chr1:81878691-81891490:+
Coord C1 exon
chr1:81878691-81878859
Coord A exon
chr1:81890140-81890273
Coord C2 exon
chr1:81891328-81891490
Length
134 bp
Sequences
Splice sites
3' ss Seq
CTTTTGACATCTACGTGTAGGAA
3' ss Score
6.3
5' ss Seq
TAGGTAAGA
5' ss Score
9.14
Exon sequences
Seq C1 exon
GCGGAGCGTTTCGGCTCTCAGCGGCTCGGGAACTGGTGTGTGGTGCGAGGCTGCGGCGGAGCCTCTACACTGAAGGCGCGAGAGGTTGGCAACTTTGATTGAAGCACATCGAGCGGCGACAGCAGTGGAAGTCATGAGCGACAGCGGCGAGCAGAACTACGGAGAGCGG
Seq A exon
GAATCCCGTTCTGCTTCCAGAAGTGGAAGTGCTCATGGATCTGGGAAATCTGCAAGGCATACCCCTGCAAGGTCTCGCTCCAAGGAAGATTCCAGGCGTTCCAGATCAAAGTCCAGGTCCAGATCTGAATCTAG
Seq C2 exon
GTCTAGATCCAGAAGAAGTTCTCGGAGGCATTATACAAGGTCACGATCTCGGTCCCGGTCCCATAGAAGATCTCGTAGCAGATCTTACAGCAGAGATTATCGAAGACGGCATAGCCACAGTCATTCTCCCATGTCTACCCGCCGGCGTCATGTTGGGAATCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000001697_MULTIEX1-2/3=C1-3
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
ENSBTAT00000002225fB7337
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TCTACACTGAAGGCGCGAGAG
R:
GCGGGTAGACATGGGAGAATG
Band lengths:
248-382
Functional annotations
There are 1 annotated functions for this event
PMID: 14709600
Presence of the CLK2 kinase prevents the usage of exons 2 (HsaEX0066781) and 3 (HsaEX0066782), generating the htra2-beta3 mRNA. The resulting HTRA2-BETA3 protein lacks the first RS domain of HTRA2-BETA1, is expressed in several tissues and has no influence on tra2-beta splice site selection. It is proposed that the regulation of the tra2-beta pre-mRNA alternative splicing provides a robust and sensitive molecular sensor that measures the ratio between HTRA2-BETA1 and its interacting proteins.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development