BtaEX0039139 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000014270 | UNC5B
Description
Bos taurus unc-5 homolog B (C. elegans) (UNC5B), mRNA. [Source:RefSeq mRNA;Acc:NM_001099029]
Coordinates
chr28:27589165-27591620:+
Coord C1 exon
chr28:27589165-27589329
Coord A exon
chr28:27589545-27589577
Coord C2 exon
chr28:27591426-27591620
Length
33 bp
Sequences
Splice sites
3' ss Seq
CCCTTTATTTCCCTCGACAGATA
3' ss Score
11.19
5' ss Seq
ACCGTAAGT
5' ss Score
10.43
Exon sequences
Seq C1 exon
TCGACGGCGGGTGGACGGAGTGGAGCAAGTGGTCAGCCTGTAGCACCGAGTGTGCGCACTGGCGCAGCCGCGAGTGCATGGCACCCCCACCCCAGAACGGAGGCCGGGACTGCAGCGGGACCCTGCTCGACTCCAAGAACTGCACTGATGGGCTGTGCGTACAAA
Seq A exon
ATAAGAAAACTCTAAGTGACCCCAAAAGCCACC
Seq C2 exon
TCCTGGAGACCTCGGGGGACATGGCGCTGTATGCGGGCCTCGTGGTGTCCATCTTCGTGGTGGTGGCCGTCCTCATAGTGGTGGCGGTGGTGGTGTACCGCCGCAACTGCCGGGACTTTGACACCGACATCACCGACTCATCCGCCGCCCTCACCGGCGGCTTCCACCCAGTCAACTTCAAGACTGCAAGGCCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014270_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.167 C2=0.030
Domain overlap (PFAM):
C1:
PF150281=PTCRA=FE(47.4=100),PF0009014=TSP_1=WD(100=85.7)
A:
PF150281=PTCRA=FE(9.5=100)
C2:
PF150281=PTCRA=PD(23.3=40.9)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCCTGCTCGACTCCAAGAA
R:
CACCACCACGAAGATGGACAC
Band lengths:
112-145
Functional annotations
There are 1 annotated functions for this event
PMID: 34381052
In the absence of Netrin-1, UNC5B induces apoptosis that is blocked upon Netrin-1 binding. Here, the authors identify an UNC5B splicing isoform (called UNC5B-delta8) expressed exclusively by ECs and generated through exon skipping by NOVA2, an alternative splicing factor regulating vascular development. UNC5B-delta8 is a constitutively pro-apoptotic splicing isoform insensitive to Netrin-1 and required for specific blood vessel development (in zebrafish) in an apoptosis-dependent manner. Like NOVA2, UNC5B-delta8 is aberrantly expressed in colon cancer vasculature where its expression correlates with tumor angiogenesis and poor patient outcome.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development