RnoEX0096620 @ rn6
Exon Skipping
Gene
ENSRNOG00000000567 | Unc5b
Description
unc-5 netrin receptor B [Source:RGD Symbol;Acc:621756]
Coordinates
chr20:30352220-30354643:-
Coord C1 exon
chr20:30354479-30354643
Coord A exon
chr20:30354254-30354286
Coord C2 exon
chr20:30352220-30352414
Length
33 bp
Sequences
Splice sites
3' ss Seq
CCCTTTATTTCCCTTGACAGATC
3' ss Score
11.98
5' ss Seq
GCCGTAAGT
5' ss Score
10.11
Exon sequences
Seq C1 exon
TGGATGGAGCGTGGACTGAGTGGAGCAAGTGGTCCGCCTGCAGCACAGAGTGTGCGCACTGGCGCAGCCGCGAGTGCATGGCACCGCCGCCCCAGAACGGAGGCCGCGACTGCAGCGGGACGCTACTTGACTCCAAGAACTGCACCGATGGGCTGTGCGTGCTGA
Seq A exon
ATCAGAGAACTCTAAACGACCCTAAAAGCCGCC
Seq C2 exon
CCCTGGAGCCGTCGGGAGACGTGGCGCTGTATGCGGGCCTCGTGGTGGCCGTCTTTGTGGTTCTGGCAGTTCTCATGGCTGTAGGAGTGATCGTGTACCGGAGAAACTGCCGGGACTTCGACACGGACATCACTGACTCCTCTGCTGCCCTCACTGGTGGTTTCCACCCCGTCAACTTCAAGACTGCAAGGCCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000567-'12-11,'12-8,13-11
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.018 A=0.167 C2=0.153
Domain overlap (PFAM):
C1:
PF0009014=TSP_1=WD(100=85.7)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGACTCCAAGAACTGCACCG
R:
CCTACAGCCATGAGAACTGCC
Band lengths:
124-157
Functional annotations
There are 1 annotated functions for this event
PMID: 34381052
In the absence of Netrin-1, UNC5B induces apoptosis that is blocked upon Netrin-1 binding. Here, the authors identify an UNC5B splicing isoform (called UNC5B-delta8) expressed exclusively by ECs and generated through exon skipping by NOVA2, an alternative splicing factor regulating vascular development. UNC5B-delta8 is a constitutively pro-apoptotic splicing isoform insensitive to Netrin-1 and required for specific blood vessel development (in zebrafish) in an apoptosis-dependent manner. Like NOVA2, UNC5B-delta8 is aberrantly expressed in colon cancer vasculature where its expression correlates with tumor angiogenesis and poor patient outcome.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]