GgaEX0006169 @ galGal3
Exon Skipping
Gene
ENSGALG00000004569 | UNC5B
Description
NA
Coordinates
chr6:12865777-12867468:+
Coord C1 exon
chr6:12865777-12865941
Coord A exon
chr6:12866516-12866548
Coord C2 exon
chr6:12867274-12867468
Length
33 bp
Sequences
Splice sites
3' ss Seq
AACTTTGTTTTTCCTAACAGATA
3' ss Score
10.6
5' ss Seq
ACCGTAAGT
5' ss Score
10.43
Exon sequences
Seq C1 exon
TGGACGGTGCATGGACGGAGTGGAGCAAGTGGTCAGCATGCAGCACCGAGTGCACCCACTGGCGCAGCCGCGAGTGCTCTGCACCGGCTCCGCGCAACGGCGGCAAGGATTGCAGCGGCGGGCTGCTCGACTCCAAGAACTGCACCGATGGGCTCTGCCTGCACA
Seq A exon
ATAAAAGAGTTCTAAGCGAACCCAAAAGCCACC
Seq C2 exon
TGCTGGAGGCCACGGGTGATGTAGCCCTGTATGCCGGTCTGGTGGTGGCCATTTTTGTCTTCATTGTCATCCTCATGGCTGTGGGGGTTGTGGTGTACCGGAGGAGGTGCCGGGACTTTGACACCGACATCACAGATTCATCAGCTGCCCTGACTGGAGGCTTCCACCCCGTCAACTTCAAGACTGCCCGGCACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000004569-'6-8,'6-6,7-8
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.015
Domain overlap (PFAM):
C1:
PF0009014=TSP_1=PD(1.9=1.8),PF0009014=TSP_1=WD(100=85.7)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCGACTCCAAGAACTGCACC
R:
CCCCCACAGCCATGAGGAT
Band lengths:
127-160
Functional annotations
There are 1 annotated functions for this event
PMID: 34381052
In the absence of Netrin-1, UNC5B induces apoptosis that is blocked upon Netrin-1 binding. Here, the authors identify an UNC5B splicing isoform (called UNC5B-delta8) expressed exclusively by ECs and generated through exon skipping by NOVA2, an alternative splicing factor regulating vascular development. UNC5B-delta8 is a constitutively pro-apoptotic splicing isoform insensitive to Netrin-1 and required for specific blood vessel development (in zebrafish) in an apoptosis-dependent manner. Like NOVA2, UNC5B-delta8 is aberrantly expressed in colon cancer vasculature where its expression correlates with tumor angiogenesis and poor patient outcome.