MmuEX0050632 @ mm9
Exon Skipping
Gene
ENSMUSG00000020099 | Unc5b
Description
unc-5 homolog B (C. elegans) [Source:MGI Symbol;Acc:MGI:894703]
Coordinates
chr10:60237714-60240302:-
Coord C1 exon
chr10:60240138-60240302
Coord A exon
chr10:60239933-60239965
Coord C2 exon
chr10:60237714-60237908
Length
33 bp
Sequences
Splice sites
3' ss Seq
CCCTTTATTTCCCTTGACAGATC
3' ss Score
11.98
5' ss Seq
ACCGTAAGT
5' ss Score
10.43
Exon sequences
Seq C1 exon
TGGATGGAGCGTGGACCGAGTGGAGCAAGTGGTCTGCCTGCAGCACAGAGTGTGCGCACTGGCGCAGCCGCGAGTGCATGGCACCGCCACCCCAGAACGGAGGCCGTGACTGCAGCGGGACGCTACTTGACTCCAAGAACTGCACTGATGGGCTGTGCGTGCTGA
Seq A exon
ATCAGAGAACTCTAAACGACCCTAAAAGCCACC
Seq C2 exon
CCCTGGAGACATCGGGAGATGTGGCACTGTACGCAGGCCTTGTGGTGGCCGTCTTTGTGGTGGTAGCGGTTCTCATGGCCGTGGGAGTGATCGTATACCGGAGAAACTGCCGGGACTTCGACACGGACATCACCGACTCCTCTGCGGCCCTCACTGGTGGCTTCCACCCTGTCAACTTCAAGACTGCAAGGCCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020099_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.009 A=0.250 C2=0.015
Domain overlap (PFAM):
C1:
PF0009014=TSP_1=WD(100=85.7)
A:
NO
C2:
NO
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGAACTGCACTGATGGGCTG
R:
TATACGATCACTCCCACGGCC
Band lengths:
128-161
Functional annotations
There are 1 annotated functions for this event
PMID: 34381052
In the absence of Netrin-1, UNC5B induces apoptosis that is blocked upon Netrin-1 binding. Here, the authors identify an UNC5B splicing isoform (called UNC5B-delta8) expressed exclusively by ECs and generated through exon skipping by NOVA2, an alternative splicing factor regulating vascular development. UNC5B-delta8 is a constitutively pro-apoptotic splicing isoform insensitive to Netrin-1 and required for specific blood vessel development (in zebrafish) in an apoptosis-dependent manner. Like NOVA2, UNC5B-delta8 is aberrantly expressed in colon cancer vasculature where its expression correlates with tumor angiogenesis and poor patient outcome.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types