DreEX0083396 @ danRer10
Exon Skipping
Gene
ENSDARG00000033327 | unc5b
Description
unc-5 homolog B (C. elegans) [Source:ZFIN;Acc:ZDB-GENE-041213-1]
Coordinates
chr13:29070510-29078480:+
Coord C1 exon
chr13:29070510-29070674
Coord A exon
chr13:29073714-29073746
Coord C2 exon
chr13:29078286-29078480
Length
33 bp
Sequences
Splice sites
3' ss Seq
GTTGCCTTTTTTTCTAACAGATA
3' ss Score
10.35
5' ss Seq
ATCGTAAGT
5' ss Score
10.44
Exon sequences
Seq C1 exon
TGGATGGAGGCTGGACCGAGTGGGCCAAGTGGTCTGCGTGTGGGACGGAGTGCACACATTGGCGCAGTCGTGAATGTCAGGCTCCACCGCCACGTAATGGAGGACGACACTGCAGCGGCAGCATGATGGAGAGCAAGAACTGCACTGAGGGATTATGTGCACGCA
Seq A exon
ATAAAAAGGTTTCTGTTGAACATACAAGCCATC
Seq C2 exon
CTCTGGGCTCTGGGACTGGTGTCGCGGTGTACGCAGGGCTCGTGGGAGCTCTGCTTCTCTGTGTGATCCTGGTGTTGTGTGTGGGGATTCTGGTCTATCGCCGGAGCTGTCGCCATCTTCACGGTGAAATCACAGATTCGTCATCAGCCCTCACTGCTGCCTTCCACCCCGGCAACTACAAACCTCCACGACAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000033327_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.018 A=0.229 C2=0.171
Domain overlap (PFAM):
C1:
PF0009014=TSP_1=PD(1.9=1.8)
A:
NO
C2:
NO
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGAGGGATTATGTGCACGC
R:
AGACCAGAATCCCCACACACA
Band lengths:
118-151
Functional annotations
There are 1 annotated functions for this event
PMID: 34381052
In the absence of Netrin-1, UNC5B induces apoptosis that is blocked upon Netrin-1 binding. Here, the authors identify an UNC5B splicing isoform (called UNC5B-delta8) expressed exclusively by ECs and generated through exon skipping by NOVA2, an alternative splicing factor regulating vascular development. UNC5B-delta8 is a constitutively pro-apoptotic splicing isoform insensitive to Netrin-1 and required for specific blood vessel development (in zebrafish) in an apoptosis-dependent manner. Like NOVA2, UNC5B-delta8 is aberrantly expressed in colon cancer vasculature where its expression correlates with tumor angiogenesis and poor patient outcome.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]