BtaEX6009015 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23666969-23670966:-
Coord C1 exon
chr2:23670841-23670966
Coord A exon
chr2:23670249-23670427
Coord C2 exon
chr2:23666969-23667059
Length
179 bp
Sequences
Splice sites
3' ss Seq
ACTGTTTTCCTTTCTTCTAGCTG
3' ss Score
11.15
5' ss Seq
CCAGTAAGT
5' ss Score
9.09
Exon sequences
Seq C1 exon
ACTCCCTTGCCAGAACAGGAGGGCCCAACTGTTGGAACAATGGGAACTTTCGAACTGATGAGCTCCAAAGATTTGGCCTACCAGATGACAATTTATGATTGGGAGCTCTTCAACTGTGTGCATGAG
Seq A exon
CTGGAGCTAATCTATCACACATTTGGAAGGCATCATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGATCTGCCTTTGTTCCCAGCCTAGCAAGCGTGTTCAGCTCTTAAAAAAATTTATTAAGATCGCAGCCCA
Seq C2 exon
CTGTAAGGAGTACAAAAATCTGAATTCCTTTTTTGCCATCGTCATGGGACTAAGTAATGTAGCTGTGAGCCGCCTGGCACTGACGTGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'40-38,'40-37,41-38=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.024 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=PU(13.0=57.1)
A:
PF0061714=RasGEF=FE(31.9=100)
C2:
PF0061714=RasGEF=FE(16.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTCCCTTGCCAGAACAGGAG
R:
CTCCCACGTCAGTGCCAGG
Band lengths:
217-396
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]