DreEX6034780 @ danRer10
Exon Skipping
Gene
ENSDARG00000079872 | rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:ZFIN;Acc:ZDB-GENE-070912-385]
Coordinates
chr9:2949369-2952761:-
Coord C1 exon
chr9:2952636-2952761
Coord A exon
chr9:2952281-2952459
Coord C2 exon
chr9:2949369-2949459
Length
179 bp
Sequences
Splice sites
3' ss Seq
TGGATGTGTGTGTGTTGCAGCAT
3' ss Score
8.63
5' ss Seq
ACAGTCAGT
5' ss Score
0.29
Exon sequences
Seq C1 exon
ACGCCGCTGCCCGAGCAGGAGGGACCGTCTGCAGGCTCCATGTCCACGTTTGAGCTCATGAGCTCTAAAGATCTGGCCCATCAGATGACCCTCTACGACTGGGAGCTCTTCCACTGTGTGCATGAG
Seq A exon
CATGAGCTGATCTACCACACGTTCGGCCGACATCATTTTAAGAAGACCACCGCCAATCTGGACCTGTTCCTGCGGCGCTTCAATGAGGTGCAGCTGTGGGTGGTGACGGAGGTCTGTCTGTGTCCCGCGCTCAGCAAACGAGTGCAGCTCCTCAAGAAGTTCATCAAGATCGCTGCACA
Seq C2 exon
CTGTAAGGAGTTTAAGAACCTGAACTCATTCTTTGCCATTATCATGGGTCTGGGAAACCCTGCGGTCTGTAGACTCAGTCAGACATGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079872-'31-34,'31-33,33-34=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.159 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=PU(13.0=57.1)
A:
PF0061714=RasGEF=FE(31.9=100)
C2:
PF0061714=RasGEF=FE(16.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCAGGAGGGACCGTCTG
R:
CTCCCATGTCTGACTGAGTCT
Band lengths:
205-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]