BtaEX6009934 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000012615 | ZEB2
Description
zinc finger E-box binding homeobox 2 [Source:HGNC Symbol;Acc:HGNC:14881]
Coordinates
chr2:52394836-52399630:+
Coord C1 exon
chr2:52394836-52394944
Coord A exon
chr2:52395882-52397851
Coord C2 exon
chr2:52399450-52399630
Length
1970 bp
Sequences
Splice sites
3' ss Seq
AATTTTAATTGTCATTTTAGGTG
3' ss Score
8.06
5' ss Seq
CAGGTAATG
5' ss Score
9.43
Exon sequences
Seq C1 exon
CACCAAATGCTGACCCAAGGAGCAGGTAATCGCAAGTTCAAATGCACAGAGTGTGGCAAGGCCTTCAAATATAAACACCATCTGAAGGAACACCTGCGAATCCACAGTG
Seq A exon
GTGAAAAACCGTACGAGTGCCCCAACTGCAAGAAACGTTTCTCCCATTCTGGTTCCTACAGTTCACACATCAGCAGCAAGAAATGTATTGGCTTAATCTCTGTAAATGGCCGAATGAGAAACAATATCAAGACAGGTTCTTCTCCTAATTCTGTTTCTTCTTCTCCTACTAATTCAGCCATTACCCAGTTAAGAAACAAATTGGAGAATGGAAAACCACTTAGTATGTCTGAACAGACAGGCTTACTTAAAATTAAAACAGAACCACTAGACTTCAATGACTATAAAGTTCTTATGGCCACACATGGGTTTAGTGGAACTAGTCCTTTTATGAATGGTGCACTTGGAGCCACCAGCCCTTTAGGAGTTCATCCGTCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGATGGAAGCCCCTTTACTTGGATTTCCCACAATGAATAGTAATTTAAGCGAGGTACAAAAGGTTCTACAGATTGTGGACAACACAGTTTCCAGACAAAAAATGGACTGCAAGGCTGAAGAAATTTCCAAGTTGAAAGGTTATCACATGAAGGATCCATGCTCTCAACCTGAGGAACAAGGAGTTACTTCTCCTAATATTCCACCCGTAGGTCTTCCAGTAGTGAGTCATAATGGTGCCACTAAAAGTATTATTGACTACACGTTAGAAAAAGTCAATGAAGCCAAAGCTTGCCTCCAGAGCTTGACTACTGACTCAAGGAGACAGATCAGTAATATAAAGAAAGAGAAGCTACGTACTTTAATAGATTTGGTCACCGATGACAAAATGATTGAGAACCACAACATATCCACTCCATTCTCCTGCCAGTTCTGTAAAGAAAGTTTTCCTGGCCCCATTCCTTTGCATCAGCATGAACGTTACCTTTGTAAGATGAATGAAGAGATCAAGGCAGTCCTGCAACCCCATGAAAACATAGTCCCCAACAAAGCTGGAGTTTTTGTTGATAATAAAGCCCTCCTCTTGTCATCTGTACTTTCTGAGAAAGGAATGACAAGCCCCATCAACCCATACAAGGACCACATGTCTGTACTTAAAGCATATTATGCTATGAACATGGAGCCCAACTCTGATGAACTGCTGAAAATTTCCATTGCTGTGGGCCTTCCTCAGGAATTTGTGAAGGAATGGTTTGAGCAACGAAAAGTCTACCAGTACTCAAATTCCAGGTCACCATCACTGGAAAGGAACTCCAAGCCGTTAGCTCCCAACAGTAACCCTCCCACAAAAGACTCTTTATTACCCAGGTCTCCCGTAAAACCCATGGACTCCATAACGTCACCATCTATAGCAGAACTCCACAACAGTGTTACGAATTGTGACCCTCCTCTCAGGCTAACAAAACCTTCCCATTTTACCAATATTAAACCAGTCGAAAAATTGGACCACTCCAGGAGTAATACTCCTTCTCCCTTAAATCTTTCCTCCACATCTTCTAAAAACTCCCACAGTAGTTCTTACACTCCAAACAGCTTCTCATCTGAGGAGCTCCAGGCTGAGCCTTTGGACCTGTCATTACCAAAACAAATGAAAGAACCCAAAAGTATTATAGCCACAAAGAGCAAAACGAAGGTTGGCAGCATAAATTTAGACCACAACAGTGTTTCTTCGTCATCTGAAAACTCAGATGAGCCTCTGAACTTGACTTTTATCAAGAAGGAGTTTTCAAATTCAAATAATCTGGACAGCAAAAGTGCTAACCCAGTGTTCGGCATGAACCCCTTTAGTGCCAAACCTCTCTACACAGCTCTTCCTCCACAAAGCGCGTTCCCCCCGGCCACTTTCATGCCACCAGTCCAGACGAGTATCCCTGGACTGCGACCATACCCAGGACTGGATCAGATGAGCTTCCTACCACACATGGCCTATACTTACCCAACTGGAGCAGCTACTTTTGCTGACATGCAGCAAAGGAGAAAGTACCAGCGGAAACCAGGATTTCAG
Seq C2 exon
GGAGAATTGCTTGATGGAGCACAAGACTACATGTCAGGCCTAGATGACATGACAGACTCCGACTCCTGTCTGTCTCGAAAGAAGATCAAGAAGACAGAGAGTGGCATGTATGCATGTGATTTGTGTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012615-'13-11,'13-10,14-11=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.189 A=0.457 C2=0.131
Domain overlap (PFAM):
C1:
PF134651=zf-H2C2_2=PU(38.5=27.0)
A:
PF134651=zf-H2C2_2=PD(57.7=2.3),PF0004624=Homeobox=WD(100=7.8)
C2:
PF134651=zf-H2C2_2=PU(38.5=16.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAATGCTGACCCAAGGAGCA
R:
TGTGTGTTCGTATTTATGTCGCA
Band lengths:
286-2256
Functional annotations
There are 3 annotated functions for this event
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000260; ELM sequence: PLNLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000261; ELM sequence: PLDLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000262; ELM sequence: PLNLT; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]