RnoEX6016712 @ rn6
Exon Skipping
Gene
ENSRNOG00000004677 | Zeb2
Description
zinc finger E-box binding homeobox 2 [Source:RGD Symbol;Acc:1307272]
Coordinates
chr3:29868356-29872984:-
Coord C1 exon
chr3:29872876-29872984
Coord A exon
chr3:29870024-29871993
Coord C2 exon
chr3:29868356-29868536
Length
1970 bp
Sequences
Splice sites
3' ss Seq
AATTTTAATTATCCTTTTAGGTG
3' ss Score
8.83
5' ss Seq
CAGGTAACG
5' ss Score
10.52
Exon sequences
Seq C1 exon
CACCAAATGCTAACCCAAGGAGCAGGTAACCGCAAGTTCAAGTGCACAGAGTGTGGCAAGGCCTTCAAGTACAAGCACCACCTGAAAGAACACCTGAGAATTCACAGTG
Seq A exon
GTGAAAAACCTTACGAATGCCCCAACTGCAAGAAACGCTTCTCTCATTCTGGTTCCTACAGTTCACATATCAGCAGCAAGAAATGTATTGGTTTAATCTCAGTAAATGGCCGAATGAGAAACAATATCAAGACGGGTTCTTCCCCTAATTCTGTTTCTTCTTCTCCTACTAACTCAGCCATTACTCAACTAAGGAACAAGTTGGAAAATGGAAAACCACTTAGCATGTCTGAGCAGACGGGCTTACTTAAGATTAAAACAGAACCACTAGACTTCAATGACTATAAAGTTCTTATGGCAACACATGGGTTTAGTGGCACTAGTCCCTTTATGAACGGTGGGCTTGGAGCCACCAGCCCTTTAGGTGTGCACCCATCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGTTAGAAGCCCCTTTACTTGGATTTCCCACTATGAATAGTAATCTGAGTGAGGTACAAAAGGTTCTACAGATTGTGGATAATACGGTTTCTAGGCAAAAGATGGACTGCAAAACTGAAGACATTTCAAAGTTGAAAGGTTACCACATGAAGGATCCATGTTCTCAGCCAGAAGAACAAGGGGTTACTTCTCCTAGTATTCCAGCCGTCGGTCTTCCAGTAGTGAGTCATAATGGTGCCACTAAAAGTATCATTGACTATACCTTAGAGAAAGTCAACGAAGCCAAAGCTTGCCTCCAGAGCTTGACTACTGACTCAAGGAGACAGATCAGTAATATAAAGAAAGAGAAGCTACGTACTTTGATAGATTTGGTCACCGATGATAAAATGATTGAGAACCACAGCATACCCACTCCGTTTTCATGCCAGTTCTGTAAAGAAAGCTTCCCAGGTCCTATCCCCCTGCATCAGCATGAACGCTACCTGTGTAAAATGAATGAAGAGATCAAGGCGGTCCTGCAACCTCACGAAAACATAGTCCCCAACAAAGCTGGGGTGTTCGTTGACAATAAAGCCCTCCTCTTGTCATCTGTACTTTCTGAGAAAGGACTGACAAGCCCGATCAACCCGTACAAGGACCACATGTCTGTACTCAAAGCATACTATGCTATGAATATGGAACCCAACTCTGATGAACTGCTGAAAATCTCCATTGCTGTGGGCCTTCCTCAGGAGTTTGTGAAGGAATGGTTTGAGCAGAGAAAAGTCTACCAGTACTCCAGTTCCAGGTCACCATCACTGGAGAGGAACTCCAAGCCCTTAGCTCCCAACAGCAACCCCACCACAAAAGACTCTTTGTTACCCAGGTCTCCTGTAAAACCTATGGACTCAATCACGTCACCATCTATAGCAGAACTCCACAACAGTGTCACGAGTTGTGATCCTCCTCTCAGGCTAACAAAATCTTCCCACTTCACCAATATTAAAGCAGTTGATAAACTGGACCACTCAAGGAGTAACACTCCTTCTCCTTTGAATCTTTCTTCCACGTCTTCTAAAAACTCCCACAGTAGCTCGTACACTCCAAATAGCTTCTCTTCCGAGGAGCTGCAGGCTGAGCCGTTGGACCTGTCATTACCAAAACAAATGAGAGAGCCCAAAAGTATTATAGCCACAAAGAACAAGACGAAAGCTACTAGCATAAGTTTAGACCACAACAGTGTTTCTTCATCCTCTGAAAACTCAGATGAACCTCTGAATTTGACTTTTATCAAGAAAGAGTTTTCAAATTCTAATAACCTGGACAATAAAAGCACTAACCCTGTGTTCGGCATGAACCCATTTAGTGCCAAGCCTTTATACACAGCTCTTCCACCACAGAGCGCATTTCCCCCTGCCACTTTCATGCCACCAGTCCAGACCAGCATCCCCGGGCTACGACCATACCCAGGGCTGGATCAGATGAGCTTCCTACCACATATGGCCTATACCTACCCAACGGGAGCAGCTACCTTTGCTGATATGCAGCAAAGGAGGAAATACCAGCGGAAACAAGGATTTCAG
Seq C2 exon
GGAGAATTGCTTGATGGAGCACAAGACTACATGTCGGGCCTAGATGACTTGACAGACTCCGATTCCTGTCTGTCTCGAAAGAAGATAAAGAAGACAGAAAGTGGCATGTATGCATGTGACTTATGTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000004677-'45-28,'45-27,46-28=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.189 A=0.443 C2=0.071
Domain overlap (PFAM):
C1:
PF134651=zf-H2C2_2=PU(38.5=27.0)
A:
PF134651=zf-H2C2_2=PD(57.7=2.3),PF0004624=Homeobox=WD(100=7.8)
C2:
PF134651=zf-H2C2_2=PU(38.5=16.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAATGCTAACCCAAGGAGCA
R:
TGTGTGTTCGTATTTATGTCGCA
Band lengths:
287-2257
Functional annotations
There are 3 annotated functions for this event
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000260; ELM sequence: PLNLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000261; ELM sequence: PLDLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000262; ELM sequence: PLNLT; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]