DreEX6019747 @ danRer10
Exon Skipping
Gene
ENSDARG00000078416 | zeb2b
Description
zinc finger E-box binding homeobox 2b [Source:ZFIN;Acc:ZDB-GENE-080717-1]
Coordinates
chr6:1159953-1167003:-
Coord C1 exon
chr6:1166895-1167003
Coord A exon
chr6:1164693-1166680
Coord C2 exon
chr6:1159953-1160055
Length
1988 bp
Sequences
Splice sites
3' ss Seq
GTTATCTCTGTTCACTGTAGGTG
3' ss Score
9.86
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
Exon sequences
Seq C1 exon
CATCAGATTCTCAACCAGGGTTCTGGGAACCGCAAGTTCAAATGCACTGAATGTGGCAAGGCCTTCAAATACAAACACCATCTAAAGGAGCACCTGCGGATACACAGTG
Seq A exon
GTGAGAAGCCATACGAATGTCCAAACTGTAAGAAGAGGTTTTCACACTCCGGGTCCTACAGTTCACACATTAGTAGTAAGAAATGCATTGGATTGATTGCAATCAACGGCAGGGTTAGGAACAACCTAAAGACTGGTTCATCCCCAACTTCTGCTTCTTCCTCTCCTACGAACAATGCCATTTCTCAGCTACGACACAAACTGGAGAACGGCAAACCTCTCGGATTGCAAGACCAGTCCAACCACCTCAACATCAAATCTGAACCACTGGACTTCAACGACTATAAGCTGATGATGGCCTCTCATGGCTACGCTACGGGAAGCCCTTTTCTCAACGGTGGAGTGAGGGGAGGCAGTCCGTTGGGCATTCATAACTCCCAGAGTCCCCTACAGCACCTGGGAATGGGTATTGAGGGGCAAATGTTGGGATACCCATCACTGGGAAACAATCTGAGTGAGGTACAGAAGGTGCTTCAGATTGTGGATAACACAGTTTGTCGACAAAAGATGGACTGCAAGCCGGAGGAGATCTCCAAGCTGAAGGCGTACATGAAGGAGTTGGGTTCCCACATAGAGGAGCAGAAGCAGGGGCTCAACTCCTCTGGTGGAGGCCAGGGCACTCTTCCACTCATCAATCACAATGGAGCCACCAAGAGCATCATAGACTACACACTCGAGAAGGTCAACGAAGCCAAAGCCTGCCTCCAAAGCTTGACTACAGACTCTAAGAGGCAAATTAGCAATATAAAGAAAGAGAAGGCAAATCACATGCTAGATTTAGGAATGGAGGAAAAAGCTCATGAGAATAATTTAATGTTTACACCATTCTCCTGCCAGTATTGCAAAGAGACGTTCCCAGGGCCCATTCCTTTACACCAGCATGAGCGTTACCTGTGCAAGATGAACGAGGAAATCAAAGCAGTGCTTCAACCCGCTCAGAATGCCCTGACCAACAAACCCGGCTTGTTTTCTGAGAAACATGGCCTGTTGCATCCTTCCATCATCCCTGAGAAGAGCCTCAATGGCCCAATCAGTCCCTACAAGGACCACATGTCAGTACTAAAAGCTTATTTCGCCATGAACATGGAGCCCAACTCCGAAGAACTACTGAAAATCTCCATAGCGGTTGGCCTTCCGCAGGAGTTTGTGAAAGAGTGGTTTGAGCAGAGGAAAGTCTTTCAGTACACCACTTCCAGAACTCCTCCTTTAGACAGGAGTCCCGTGGAGTCTATCCATCCGGTCTCCGCTCACACACCCACTAAAGACTCTTTAGGAATTCGTTCTCCGATGTCCCTTGTCAAAGGTAGCGACCGCATCACATCCCCAGCCATCCCCGAGCTACATAACAACTGCGACACCCCACTCAGGCTGTCCAAAACCCCCCAATACTCCAATCACAAGCAGCTAGGGGATAAGATGGACCACTCCCGGAGCAACACGCCCTCTCCTCTGAACTTATCGTCCGCCTCGTCCAAAAATTCCCACACTAGCTCTTACACGCCCAACAGCTTCACCTCCGAGGACCTGCAGGCCGAGCCTCTTGACCTCTCCTTGCCAAAGCTCATGAAGGAGCCAAAGCACATCTTGACTGTGAAAAGCCGACTTAAGCTCAACAGTGGCCCCATGGACCACAATCACGTAGCGACACCCCGCGAGCATGCGGACGAGCCACTCAACTTGGCGTATCTCTCCAAGAAGGAGTTCGGCAGCCCCAATGCAAACAGCAATCTGGACAAAAGCTCTAGTCCTATGTTTGGTCTAAACCCGTTCGCTGCCAAACCAATGTACACCTCCCTGCCACCCCAAAGTGCATTCCCGCCGCCTACATTCATGCCCCCAGTGCAGGCCAGCCTCCCAGGGCTCAGACCCTATCCCAGTCTCGACCAGATAAGCTTTTTGCCACACATGGCCTACACGTACGCGGCAGGGGCAGCCAGCTTCGCAGAGATGCAGCAAAGGAGAAAATATCAGCGGAAACCAGGGTTCCAG
Seq C2 exon
AGCGAGCTTCTTGACGGGCCGGCAGATTATCTGAGCAGTCTAGATGATATGGCCGACCCCGAGGCCTGTCTGTCCAGGAAGAAGATTAAGAAAACAGAAAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000078416-'5-6,'5-5,6-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.216 A=0.468 C2=0.205
Domain overlap (PFAM):
C1:
PF134651=zf-H2C2_2=PU(38.5=27.0)
A:
PF134651=zf-H2C2_2=PD(57.7=2.3),PF0004624=Homeobox=WD(100=7.5)
C2:
PF134651=zf-H2C2_2=PU(27.3=17.1)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATCAGATTCTCAACCAGGGT
R:
TCTGTTTTCTTAATCTTCTTCCTGGA
Band lengths:
207-2195
Functional annotations
There are 3 annotated functions for this event
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000260; ELM sequence: PLNLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000261; ELM sequence: PLDLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000262; ELM sequence: PLNLT; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]