BtaEX6016725 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021900 | PKN2
Description
protein kinase N2 [Source:HGNC Symbol;Acc:HGNC:9406]
Coordinates
chr3:55268426-55286769:-
Coord C1 exon
chr3:55286624-55286769
Coord A exon
chr3:55286325-55286538
Coord C2 exon
chr3:55268426-55268611
Length
214 bp
Sequences
Splice sites
3' ss Seq
TTATCTTTTCCCTTTGTAAGGCT
3' ss Score
5.99
5' ss Seq
CAGGTATGT
5' ss Score
9.8
Exon sequences
Seq C1 exon
CCAAACCTGTCATAAGTCCTCTTGAACTTCGAATGGAAGAATTAAGGCATCATTTTAGGATAGAGTTTGCAGTAGCAGAAGGTGCAAAGAATGTAATGAAATTACTTGGCTCAGGAAAAGTGACAGACAGAAAAGCACTTTCAGAG
Seq A exon
GCTCAAGCAAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTGAAGTATTCATTAGAGCAGAGATTAAATGAACTTCCCAAGAATCATCCCAAAAGCAGTATTATTATTGAGGAGCTTTCACTTGTTGCATCACCAACACTAAGTCCACGTCAAAGTATGATATCTACCCAAAACCAATATAGTACCCTGTCCAAACCAGCAGCACTAACAG
Seq C2 exon
GTACTTTGGAAGTTCGTCTTATGGGCTGTCAAGATATCCTAGAGAATGTCCCTGGACGGTCGAAAGCAGCATCAGTTGCACTACCTGGTTGGAGTCCAAGTGAGACCAGATCATCCTTCATGAGCAGAACAAGTAAAAGTAAAAGCGGAAGTAGTCGAAATCTACTAAAAACTGATGACTTATCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021900-'12-13,'12-9,13-13=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.061 A=0.139 C2=0.238
Domain overlap (PFAM):
C1:
PF0218511=HR1=PU(53.4=79.6)
A:
PF0218511=HR1=PD(43.8=44.4)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTCCTCTTGAACTTCGAATGGA
R:
AGATTTCGACTACTTCCGCTT
Band lengths:
295-509
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]