BtaEX6021154 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000006828 | RAPGEF3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:HGNC Symbol;Acc:HGNC:16629]
Coordinates
chr5:32705499-32706034:+
Coord C1 exon
chr5:32705499-32705559
Coord A exon
chr5:32705646-32705722
Coord C2 exon
chr5:32705888-32706034
Length
77 bp
Sequences
Splice sites
3' ss Seq
ATTTGTGCTGCTTCTCTCAGTGT
3' ss Score
7.86
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
GTGAAGCGGGAACTGGCTGCAGTCCTGCTCTTTGAACCGCACAGCAAGGCAGGAACTGTGT
Seq A exon
TGTTCAGCCAGGGGGACAAGGGCACCTCGTGGTACATTATCTGGAAGGGATCGGTCAACGTGGTGACCCATGGCAAG
Seq C2 exon
GGCCTGGTGACCACCCTGCACGAGGGAGATGACTTCGGACAGCTGGCTCTGGTGAACGATGCACCTCGGGCCGCCACCATCATCCTGGGAGAAGACAACTGTCATTTTCTTCGTGTGGACAAGCAGGACTTCAACCGTATCATCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006828-'10-12,'10-11,11-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=PU(8.2=33.3)
A:
PF0002724=cNMP_binding=FE(29.4=100)
C2:
PF0002724=cNMP_binding=FE(56.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCTTTGAACCGCACAGCAAG
R:
ACGGTTGAAGTCCTGCTTGTC
Band lengths:
172-249
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]