BtaEX6063080 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66399820-66406176:-
Coord C1 exon
chr14:66406110-66406176
Coord A exon
chr14:66402663-66402804
Coord C2 exon
chr14:66399820-66399979
Length
142 bp
Sequences
Splice sites
3' ss Seq
TTTCCTACACTACATTTCAGCTA
3' ss Score
4.94
5' ss Seq
CAGGTAAGC
5' ss Score
9.88
Exon sequences
Seq C1 exon
CGGGTGCCTTAGCGAGGCTGGTACGGTGTGTGGCTGGGTCCTCTGCACGGCTTGGGGTCTGAACCAG
Seq A exon
CTATGGCCACCAAAGATTATCCATCATTGTGGGGCTTCGGGACAACAAAAACATTTAAAATTCCTGTCGAACATTTGGATTTCAAGTATATTGAAAAATGTTCAGATGTTAAACACTTGGAAAAAATTCTTTGTGTGCTCAG
Seq C2 exon
GTCTGGTGAGGAAGGATACTATCCTGAACTTACTGAATTTTGTGAAAAGCACCTTTCAGGCTTGGCTCCTGGAAGTAGAGCTTTGAGGAAAGATAAACCTGCGGCAACAGCATCCAGTTTTACAGCAGAAGAATGGGAAAAAATTGACGGTGACATAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544-'0-3,'0-1,1-3=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref, Alt. ATG (>10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.000 C2=0.111
Domain overlap (PFAM):
C1:
NA
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTAGCGAGGCTGGTACGGT
R:
TTTTTCCCATTCTTCTGCTGTAAA
Band lengths:
202-344
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]