BtaEX6063081 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66381610-66399979:-
Coord C1 exon
chr14:66399820-66399979
Coord A exon
chr14:66387784-66387909
Coord C2 exon
chr14:66381610-66381674
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTTTTCTCTCTTCTTCTCAGAGT
3' ss Score
11.15
5' ss Seq
AAGGTACAC
5' ss Score
7.56
Exon sequences
Seq C1 exon
GTCTGGTGAGGAAGGATACTATCCTGAACTTACTGAATTTTGTGAAAAGCACCTTTCAGGCTTGGCTCCTGGAAGTAGAGCTTTGAGGAAAGATAAACCTGCGGCAACAGCATCCAGTTTTACAGCAGAAGAATGGGAAAAAATTGACGGTGACATAAAG
Seq A exon
AGTTGGGTATCAGAAATTAAAAAAGAAGATGATAAAATACACTTCCATGAAACTGAAACATTTCCAGCAGTGGAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCCATCTTCATGTTGCCAAG
Seq C2 exon
GAAAAGAATTCTAAAAGTAGACCAGCTAAAAAGAAAATTCCGAGAGATTATGCAGAGTGGGATAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544-'5-5,'5-4,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.111 A=0.524 C2=0.545
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCTGAACTTACTGAATTTTGTGAA
R:
TCCCACTCTGCATAATCTCTCGG
Band lengths:
202-328
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]