BtaEX6076830 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021273 | SEMA4B
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:HGNC Symbol;Acc:HGNC:10730]
Coordinates
chr21:22036087-22037917:+
Coord C1 exon
chr21:22036087-22036237
Coord A exon
chr21:22037505-22037715
Coord C2 exon
chr21:22037802-22037917
Length
211 bp
Sequences
Splice sites
3' ss Seq
ACCAACGCCTTCTCCTTCAGTGC
3' ss Score
8.5
5' ss Seq
CTGGTGCGT
5' ss Score
8.29
Exon sequences
Seq C1 exon
GAACGGGGGGACCACGGAGGGCTCTGCCGTCTGTGTCTTCACCATGAAGGACGTGCAGTCCACCTTCAACGGCTTCTACAAGGAGGTGAACCGCGAGACGCAGCAGTGGTACACAGTGACCCACCCAGTACCCTCGCCCCGGCCGGGCGCG
Seq A exon
TGCATCACCAATAGTGCCCGGGAGAGGAAGATCAACTCGTCACTGCAGCTCCCTGACCGCGTGCTGAACTTCCTCAAGGACCACTTCCTGATGGACGGACAGGTCCGCGGCCGCATGTTGCTGCTGCAGCCCCAGGCTCGCTACCAGCATGTGACTGTCCACCGTGTGCTGGCCCTGAACCGCACCTACGACGTCCTCTTCCTGGGCACTG
Seq C2 exon
GTGACGGCTGGGTGCACAAGGCGGTGAGCGTGGGCACCGCGGTGCACATCATTGAAGAGCTCCAGGTCTTCCCGGCAGGACAGCCCGTGCGGAACCTGCAACTGGACGCCGACAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021273-'12-15,'12-14,14-15=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.137 A=0.056 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.4=100)
A:
PF0140314=Sema=FE(16.0=100)
C2:
PF0140314=Sema=FE(8.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCACGGAGGGCTCTGC
R:
GGCGTCCAGTTGCAGGTTC
Band lengths:
252-463
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]