RnoEX6003461 @ rn6
Exon Skipping
Gene
ENSRNOG00000025167 | Sema4b
Description
semaphorin 4B [Source:RGD Symbol;Acc:1307872]
Coordinates
chr1:142007234-142008587:+
Coord C1 exon
chr1:142007234-142007384
Coord A exon
chr1:142008152-142008362
Coord C2 exon
chr1:142008472-142008587
Length
211 bp
Sequences
Splice sites
3' ss Seq
TCATGCTTCTGCCTTTTCAGTGT
3' ss Score
9.25
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
Exon sequences
Seq C1 exon
GCACAGAGGGACCACAGAAGGCTCTGCCATCTGCGTCTTCACCATGAATGACGTGCAGAAGGCCTTTGATGGTCTGTATAAGAAAGTGAACAGAGAGACACAGCAGTGGTATACTGAGACCCACCCAGTGCCCACTCCCCGGCCGGGAGCG
Seq A exon
TGTATCACCAACAGTGCCCGGGAACGGAAGATCAACTCGTCGCTGCAGCTCCCGGACCGAGTGCTGAACTTCCTCAAGGATCACTTCTTGATGGATGGGCAGGTCCGCAGCCGCCTGCTGCTGCTCCAGCCACAAGCCCGCTACCAGCGTGTGGCCGTGCACCGAGTGCCTGGCCTGCACAGCACGTATGATGTACTATTTCTGGGCACTG
Seq C2 exon
GTGATGGTCGCCTGCACAAGGCAGTGACCCTGGACTCCAGAGTGCACATCATTGAGGAGCTGCAGGTCTTCCCTCAAGGACAGCCTGTGCAGAACCTGCTCTTGGACAGCCATGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025167-'11-13,'11-12,12-13=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.216 A=0.056 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.4=100)
A:
PF0140314=Sema=FE(16.0=100)
C2:
PF0140314=Sema=FE(8.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACAGAGGGACCACAGAAGGC
R:
GCTGTCCAAGAGCAGGTTCTG
Band lengths:
260-471
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]