DreEX6065716 @ danRer10
Exon Skipping
Gene
ENSDARG00000074414 | sema4ba
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4Ba [Source:ZFIN;Acc:ZDB-GENE-070705-31]
Coordinates
chr18:25801416-25808564:-
Coord C1 exon
chr18:25808414-25808564
Coord A exon
chr18:25805478-25805688
Coord C2 exon
chr18:25801416-25801531
Length
211 bp
Sequences
Splice sites
3' ss Seq
TTTTCCCCATCTTCCCACAGTGC
3' ss Score
11.4
5' ss Seq
CAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GTACAAAGGTGCTTCAGGGAGCTCAGCTGTATGTGCTTTCACTATGGACCAAGTTGAAGAGGCGTTTAATGGACGTTACCGGGAAGTTAATCGCGAGACGCAGCAGTGGTACACCTACAACCATCCAGTGCCAGAACCCAGACCTGGAGCC
Seq A exon
TGCATCACTAACACGGCCCGCGCCCAGGGGATCTCCTCTTCCCTGCACATGCCTGATAGAGTCCTGAACTTTGTGAAAGACCACTTCCTCATGGATAATGTGATTCGCAGTCAGCCTCTGCTGTTGAAACGAAATGTGCGCTACACGCAGCTCGCTGTGCACAGAGTACAGGCTCTCCACAAGACCTATGATGTGCTTTTCATTGGCACAG
Seq C2 exon
ATGACGGCAGAATCCACAAAGCCATCAACGTTAAAAACAAGATGTATATCATTGAAGAGCTGGTGATTTTTCCTGAGCCTCAATCCGTGCAGCACATCGAACTGGATGATGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074414-'14-15,'14-14,15-15=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.137 A=0.042 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.3=100)
A:
PF0140314=Sema=FE(15.8=100)
C2:
PF0140314=Sema=FE(8.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTACAAAGGTGCTTCAGGGAGC
R:
TCATCCAGTTCGATGTGCTGC
Band lengths:
260-471
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]