BtaEX6105374 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000033398 | HKDC1
Description
hexokinase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:23302]
Coordinates
chr28:25766927-25771295:+
Coord C1 exon
chr28:25766927-25767022
Coord A exon
chr28:25768823-25768922
Coord C2 exon
chr28:25771112-25771295
Length
100 bp
Sequences
Splice sites
3' ss Seq
TGGGACTTGTGTTCCAACAGGAC
3' ss Score
9.42
5' ss Seq
TTGGTAACT
5' ss Score
4.27
Exon sequences
Seq C1 exon
GGTATCCTGCTTTCATGGACGAAGAAGTTTAAGGCGAGGGGAGTTCAGGGCACAGACGTAGTGAGCTCTCTGACCAATGCTATGAAAAGACACCAG
Seq A exon
GACTTAGATGTGGACATCCTGGCTTTGGTCAACGACACCGTGGGGACCATGATGACGTGTGCCTATGACGATCCCTACTGCGAAGTTGGTGTCATCATTG
Seq C2 exon
GTACCGGCACCAACGCGTGCTACATGGAGGACATGAGCAACATCGACCTGGTGGAGGGCGACGAGGGGAGGATGTGCGTCAACACCGAGTGGGGAGCCTTCGGGGACGACGGGGCCCTGGAGGACATCCGCACCGAGTTTGACCGGGAGCTGGACCTCGGCTCTCTTAACCCCGGGAAGCAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000033398-'4-6,'4-4,5-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.193
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(15.0=100)
A:
PF0034916=Hexokinase_1=PD(11.2=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
C2:
PF0372711=Hexokinase_2=FE(25.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTAAGGCGAGGGGAGTTCAGG
R:
TGCTTCCCGGGGTTAAGAGAG
Band lengths:
249-349
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]