BtaINT0013085 @ bosTau6
Intron Retention
Gene
ENSBTAG00000017258 | ACSL3
Description
acyl-CoA synthetase long-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:3570]
Coordinates
chr2:111871799-111872729:+
Coord C1 exon
chr2:111871799-111871923
Coord A exon
chr2:111871924-111872658
Coord C2 exon
chr2:111872659-111872729
Length
735 bp
Sequences
Splice sites
5' ss Seq
AGGTAAAGG
5' ss Score
-7.53
3' ss Seq
AACCTGTTTTCTCTTTGTAGTCT
3' ss Score
8.54
Exon sequences
Seq C1 exon
GGAGAAAGATGTTTACATTGGATATTTGCCTCTGGCCCACGTCTTAGAATTAAGTGCTGAGCTTGTCTGTCTCTCCCATGGATGCCGCATTGGCTACTCTTCACCACAGACTTTAGCAGATCAGG
Seq A exon
TAAAGGAATTTCTGTGATTTGAGATACTGCTGCTGCTGCTGCTAAGTCGCTTCAGTCATGTCCAACTCTGTGCGACCCCATAGACGGCAGCCCACCAGGCTCCCCCGCCCCTGGGATTCTCCAGGCAAGAACACTGGAGTGGGTTGCCATTTCCTTCTCCAGTGCATGAAAGTGAAAAGTGAAAGTGAAGTCGCTTGGTCGTGTCTGACTCTTAGCGACCCCATGGACTGCAGCCTACCAGGCTCCTCCATCCATGGGATTTTCCAGGCAAGAGTACTGGAGTGGGTTGCCATTGCCTTCTCCTTGAGATACTAAGAAACCATAATGATCTTTAACACAATTTTCATTCAGTATGACAGTATACTATAAGGATAACAGTTAATCATTTCAAAAGAACTTGCCTTTTAAGGTCTATTTAGAATATGAGTGGATTAAAATGGGCTTCCCTTGTGGCTCAGCTGGTAAAGAATCTGCCTGCAATGTGGGAGACCTGGGTTCGATCCCTGAGTTAGGAAGATTCCCTGGAGAAGGAAAAGGCTACCCACTCCACTATTCTGGCCTGGAGAATTCCACGGACTGTATAGTCCACGGGGTCACAAAGAGTTGGACCCGACTGAGCGACTTTTCATGTCACATCACAGATTAAAATTACAGAATTTTGAAATATATTTTTTAAGTGTTTCATTGAGTTTTAAAATAATGATCACTTTATATAAACCTGTTTTCTCTTTGTAG
Seq C2 exon
TCTTCAAAATAAAAAAAGGAAGCAAAGGAGATACATCCATGTTGAAACCAACACTGATGGCAGCTGTCCCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000017258:ENSBTAT00000022939:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(8.8=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(4.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGATGTTTACATTGGATATTTGCC
R:
GGACAGCTGCCATCAGTGTTG
Band lengths:
190-925
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]