DreINT0025516 @ danRer10
Intron Retention
Gene
ENSDARG00000014674 | acsl3b
Description
acyl-CoA synthetase long-chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492]
Coordinates
chr2:47649417-47650541:+
Coord C1 exon
chr2:47649417-47649540
Coord A exon
chr2:47649541-47650469
Coord C2 exon
chr2:47650470-47650541
Length
929 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAA
5' ss Score
7.09
3' ss Seq
TCATATTTTTCTCCTCACAGTCA
3' ss Score
9.36
Exon sequences
Seq C1 exon
TGAGAATGACACCTATATTGGCTATCTGCCTCTCGCTCATGTCCTGGAGCTCAGCGCTGAGCTGGTGTGTGTGTCACACGGCTGTCGGATTGGATATTCATCACCTCAGACTCTTGCTGACCAG
Seq A exon
GTACAAACACGCACACATTAGTCATGCTGTTGTGTGGTCATCACCACGTCAAGAACTTACAATCTCACAATGCTTTAGATTAACAATGTGTTGACTTGCATTACTGCAGACAACGATATCACTGCTCTATTTATGTGCATTTGGCGAATGCCCTTCCAGCCGCAACCCATCTCTGGGAAACATCCACACAATTTAGCCTACCCAATTCACCTATACCGCATGTCTTTGGACTGTGGGGGAAACCGGAGCCCATACCCATGCGAACGTAGGGAGAATATGGAAACTCCACACAGAAACGCCAACTGAGCCGAGGCTTGAACCAGCGACCTTCTTGCTGAGGCGACAGCATTACCTACTGCGCCACTGCTTCGCCGTTTTTAAGAAAATGTTAAATGTAATTGAGTCCTATTACATTATTCCTTCACAAGCCCATTTCAGCCAGACAATACTTCCTGAATTCAGATCCACTGAAACGGCGGGTGACCATCACATCAAGCAGATCAGTGCTGTCTGAAAAAAAGGTGGACACTCATATTTCTCAAAATAAAAAATATTACACTACAGAATATTACACTTCATTTTTTATAAGGAGCTTTTTGGTTTGGGCTATAAAGGAGGATTTGTTTTATTTGCTGCTTTGAAAAAAAACCCAGAAGTTTTTCTTTCTTTCTTTTTTTTCTGCTGTATTGAAAACTTATCAAACCGTGACACCACTGTGTTGCATCGTACTGAACCATGATTTTTGTGAACTTTACACCCCAAATATTTATAGTTATTAGACTGGATCAACAGATACTATTTTTGGAACACCCTCAAAATGAATGAGAAACAAATATTGGATTTCATTCAGTGAAAAGTGATGCAGGGGAGTAAAATAATGCAGAGTGAATCAAAGATTGTAATACTATATCATATTTTTCTCCTCACAG
Seq C2 exon
TCATCTAAAATTAAAAAGGGCAGTAAAGGAGACACCAGCGTCCTCAAACCAACACTGATGGCAGCTGTTCCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014674:ENSDART00000141974:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.024 A=NA C2=0.208
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(8.6=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(4.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGACACCTATATTGGCTATCTGC
R:
GGAACAGCTGCCATCAGTGTT
Band lengths:
190-1119
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]