BtaINT0013096 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018986 | ACSL4
Description
acyl-CoA synthetase long-chain family member 4 [Source:HGNC Symbol;Acc:HGNC:3571]
Coordinates
chrX:62503660-62504074:-
Coord C1 exon
chrX:62503924-62504074
Coord A exon
chrX:62503784-62503923
Coord C2 exon
chrX:62503660-62503783
Length
140 bp
Sequences
Splice sites
5' ss Seq
GGGGTAAGA
5' ss Score
7.83
3' ss Seq
TTTCTCTTACTTTGTTTTAGACC
3' ss Score
10.85
Exon sequences
Seq C1 exon
TGAGCGTTTCTCCAAATAGACCAACTCCTTCAGACTTGGCTATTGTCATGTATACCAGTGGATCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGGAATGACTGGCCAGTGTGAGAGAATTCCTGGGCTGGG
Seq A exon
GTAAGAGAAAATTATTGAGTGTTCTTAGAACTAATGTTTGACATTCTCTAAACTCAGACTATAGTAAAACAATAATTGATGTTTCTAATTTACAATGTCTTTTTAAGAATGGCTAGAATCTTTCTCTTACTTTGTTTTAG
Seq C2 exon
ACCAAAGGATACGTATATTGGCTACCTGCCTTTGGCTCATGTGCTTGAGTTGACAGCAGAGATCTCTTGCTTTACCTATGGCTGTAGGATTGGATATTCTTCTCCACTTACACTCTCTGACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018986:ENSBTAT00000054636:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.098 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(10.5=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(8.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCAACTCCTTCAGACTTGGC
R:
TGGTCAGAGAGTGTAAGTGGAGA
Band lengths:
255-395
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]