MmuINT0010181 @ mm10
Intron Retention
Gene
ENSMUSG00000031278 | Acsl4
Description
acyl-CoA synthetase long-chain family member 4 [Source:MGI Symbol;Acc:MGI:1354713]
Coordinates
chrX:142343074-142343457:-
Coord C1 exon
chrX:142343307-142343457
Coord A exon
chrX:142343198-142343306
Coord C2 exon
chrX:142343074-142343197
Length
109 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGA
5' ss Score
6.65
3' ss Seq
TTTCTCTTACTTTGTTTTAGACC
3' ss Score
10.85
Exon sequences
Seq C1 exon
TGAGCGTTCCTCCAAGTAGACCAACCCCTTCAGACATGGCCATTGTCATGTACACCAGTGGTTCTACGGGCCGCCCCAAGGGAGTGATGATGCATCATAGCAATTTGATTGCTGGAATGACAGGCCAGTGTGAACGTATCCCTGGACTAGG
Seq A exon
GTGAGAGATGCTCATTCAACTTTATTAGAACTCCAGAGTTCATGATTAAGTTCTTTGTTTATGATGCCTTTGCACGAGTGGTTTGCATGTTTCTCTTACTTTGTTTTAG
Seq C2 exon
ACCGAAGGACACATATATTGGCTACTTACCTTTGGCTCATGTGCTGGAACTGACAGCAGAGATATCATGCTTTACCTATGGCTGTAGGATTGGATACTCTTCACCCCTTACACTGTCTGACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031278:ENSMUST00000033634:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.235 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(10.5=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(8.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCCTTCAGACATGGCCATTG
R:
TGGTCAGACAGTGTAAGGGGT
Band lengths:
250-359
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types