BtaINT0050528 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018560 | DNAH3
Description
Bos taurus dynein, axonemal, heavy chain 3 (DNAH3), mRNA. [Source:RefSeq mRNA;Acc:NM_001099199]
Coordinates
chr25:18953921-18956201:-
Coord C1 exon
chr25:18956002-18956201
Coord A exon
chr25:18954138-18956001
Coord C2 exon
chr25:18953921-18954137
Length
1864 bp
Sequences
Splice sites
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
3' ss Seq
CACCTTTGTGTCCTCAACAGGGT
3' ss Score
10.16
Exon sequences
Seq C1 exon
GGAGAAAGAACTAATGAAATCTGCTTTTCATTAGCCATGAGTCCAATTGGGGACGCCTTCCGGAACCGCCTGCGAATGTTCCCTTCTCTGATCAATTGCTGCACAATTGATTGGTTCCAGTCTTGGCCCACAGATGCCCTGGAGTTGGTGGCCAACAAATTTCTAGAAGATGTAGAGCTCGATGACAATATTCGGATACA
Seq A exon
GTAAGTGCTAGCTGCATTTTAGTACCCCAAACAAATATTTCAAATGAAAATCAAGATATTGGCTCTATTCTTTTTGCTGTTTTAAGATTTATCAATTTATGTATGTTCTGTTCTGTTCAGTCACTAAGTCGTGTGTCTGACTCTTTGTGACTGCATGGACTGCTGCATGCTTGACTTCCCTGTCCTTCACTATCTCCCAGAGTGTCCACTGAGTTGGGGATGCTATCTAACCATCTCATCCTCTGCCTCCCCCTTCTCCTTTTGCCTTCAGTCTTTCCCAGAATCAAGGTCTTTTTCAATGAGGAATAAATTATAAAAAATAAATACCATTTAGATATTTAATAAAACACCTTTGCTGAAAAAGAAGAATATTTCACTGTTCAGAATTGTTCTAGAGATGTACTGTTGTCTATGTACTGTGGTCCAGCTATGAGAACCTGGATTTCAATAATCTTAGAGCATTCATTCTCAACAGGGGGGATATTGCTGCCTAGGGGATGGTACTTGTGAGGGATTTTGTTGTGAAAAAAAAATATTTTGTGTGTAAAGCACAGACACACAAAATAAAGCACTGAATTTTGGCTAGAGTAAGAGAAAGGATAAGACTGTTGACCATTCTAAGTGTCCTATGAAGGTAAGGGTCATGTCACCTGCCTTGTATGGTTCTGTATCCTTAATACCTAGTACATAGTAGGTTCAAATCCTGCTCATTAAAGGAGTAAATAGAAAATGAAGAAGTAGGTAGTGGTCAGAATGTTCATTGCTTTAAATTCCATTTTAAGATCTTTGGACTTTGTTCTCTAGGCCCAGGAATGCTTAACCTGGGTCTATAGGCTCATGACCAGAACTCAGAGGGTCAGTGAATGTGAGTGGGGGAAAGTGCATCTTCGTTTTCATTGACATCTAACTGGCACTTTGCATTTCTTTCAATGATGAACATAGGCAATAAACCCCATAGGATTGTGTAGGACCTGTGACTTTGTTACCAGTAGGCACTGCAATTATTTTCATAACTCGTTCAGTCATTACAGATGCACCAATTTATCATCCCCTTCAAAATGATGGTAGTTTTCAGACCTGTTGTCCAGTCTTACTATTTAATGCTGTAGTTGGCAAACTATGATCCACGGCTGAGTCCAGTTCACAGCCTTATTTTTGCAAATATGCTTTATTGGAAAACAGCCATGCACACTACAGAGGCAGAGTTGAATAGTTGCTACTTGAGACCATGTGGCTCACAAAGCCTAAAATAGTTACTATCTGATCCTAACCAGAAAAAGTCTGTTGACCCGTTATTTAACATGTAAATGAAATCACACATATTGAGGCTTACCTGCAATAGTTAAGAATCCATGCTTCCAGTGGATTCTTAACCACTGCAGGCTGTGTGGGTTCAATCCAGGTCAGGAAACTAAAATCCTACATGCCACAAGGCACAGCCAAACACATGCACACACACATACACATTACTATATCATAAATTTGGTGTTTAAAATATTTAGAAAAATCTTTAACATAATTGGTTTCTTTTGTAATTCTATAATATATTATTTTATGCATTAAAATATTATTCTGAAAAGTCCAGAGGCTTCATCAGACTGTCAAAGATTAAAAATTCCTGCTTAAGCCCTGGTGATTCACAAAAGTGATTAAAGCTGGAAATTGCCTGATCAAAGTTCATTCTAGTAAGACTATATACCTCCCCAATCCTTCTTGAGGCTCTGTGGACATGCTTCAAAGAACTGGGGGTTCTAGCTGGCCAATGTGAGTGGCTTTCTCACATCCTTACTTCTGTGAACCAAAGCTGTGCTAGATCTCTCTGGAGCAAGTTGGGCCTGTCTGACCACCTTTGTGTCCTCAACAG
Seq C2 exon
GGTCATCTCCATGTGCAAATATTTCCAGGAGAGTGTGAAAAAGCTGTCACTTGATTATTACAACACCCTTCGAAGGCACAACTATGTTACCCCCACCTCCTACCTCGAATTGATCCTAACATTCAAGACACTCCTGAATAAGAAGAGGCAAGAGGTTGATATGATGAGGAACCGATATCTGACAGGCCTGCAAAAACTTGAATTTGCAGCTTCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018560:ENSBTAT00000024702:49
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF127802=AAA_8=FE(24.3=100)
A:
NA
C2:
PF127802=AAA_8=PD(16.5=61.6),PF127772=MT=PU(4.3=20.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCCATGAGTCCAATTGGGGA
R:
GGCCTGTCAGATATCGGTTCC
Band lengths:
356-2220
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]