BtaINT0059575 @ bosTau6
Intron Retention
Gene
ENSBTAG00000032509 | FAM166B
Description
Bos taurus family with sequence similarity 166, member B (FAM166B), mRNA. [Source:RefSeq mRNA;Acc:NM_001038513]
Coordinates
chr8:60197769-60198469:-
Coord C1 exon
chr8:60198377-60198469
Coord A exon
chr8:60197993-60198376
Coord C2 exon
chr8:60197769-60197992
Length
384 bp
Sequences
Splice sites
5' ss Seq
GGGGTAAGA
5' ss Score
7.83
3' ss Seq
GGGTGAGACTTGTGCTGCAGGTA
3' ss Score
2.87
Exon sequences
Seq C1 exon
AAGAGAAACAAAAGGAACTGCCTGGGTCCCCTTGGCTATGGCCAGCACCTTCATACCAGGACTGAACCCCCAGAACCCTCATTATATCCCGGG
Seq A exon
GTAAGAGCATACCCGTTGCTTCTGGGAGTAGGGGTAATAAGGATATAGAGTTGTGCCACCTCGGCCTTGGGGGCCAAGTGGACATTGTGGTATGGGCGGCTTATGGATGGGTTATCTATGATGTCTATGATGCTGGATGGAAAAGGAAAAGCAAAGGAATAGTAGGAACCGGGCCTGCTGTGAAGCTTTGTGTTGTACTCTCCATGGCAACAGGGTAACAGCCGAGGGGAAGGTTGAAAACCCAGACAGAGAACTAAGAAATGCCTGCTCCAAATATACACACAGTTGAGTCCAGAATGAGCAGGGCCTCTGCGGTCTCTGCTCCTCTGTCTGGGGATGGTGAGGCGGGGGGCTCGAGGTGAGCGGGTGAGACTTGTGCTGCAG
Seq C2 exon
GTACACTGGACACTGCCCACTACTTCGGTTCAGCATGGGCCAGACCTACGGACAGATGACTGGGCAGCTACTTCGCGGCTCTCCTGGCCTCGCCTGGCCCCCTGCCCACCGCACACTTCTGCCTCCCATTCAGCCTCCAAGATCCCCCGAGCCTCGCAGGAGAAGCCTGCCTGTCAGGCCTGGACACGAAAGGCTCAGCTCCAGCATGGTCCCCGGGTACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032509:ENSBTAT00000046106:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.105 A=NA C2=0.658
Domain overlap (PFAM):
C1:
PF106294=DUF2475=PU(9.5=31.6)
A:
NA
C2:
PF106294=DUF2475=PD(88.9=73.7),PF106294=DUF2475=PU(13.4=11.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGAGAAACAAAAGGAACTGCC
R:
GACCATGCTGGAGCTGAGC
Band lengths:
304-688
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]