BtaINT0070148 @ bosTau6
Intron Retention
Gene
ENSBTAG00000000704 | GUSB
Description
Bos taurus glucuronidase, beta (GUSB), mRNA. [Source:RefSeq mRNA;Acc:NM_001083436]
Coordinates
chr25:28172369-28173084:-
Coord C1 exon
chr25:28172899-28173084
Coord A exon
chr25:28172551-28172898
Coord C2 exon
chr25:28172369-28172550
Length
348 bp
Sequences
Splice sites
5' ss Seq
GTGGTCAGT
5' ss Score
4.85
3' ss Seq
TTGTCTCCCTGTATCTGCAGTGG
3' ss Score
8.62
Exon sequences
Seq C1 exon
TCGGGCCCCACCCTGGACATGCCGGTGCCCTCCAGCTTCAACGACGTGGGCCAGGATGGGCAGCTGCGGAGTTTTGTCGGCTGGGTGTGGTATGAACGGGAGATCACCTTGCCCCAGCGGTGGACCGAAGACCTGGGCACAAGAGTGGTTCTGAGGATCGGCAGCGCCCACTACTATGCCATTGTG
Seq A exon
GTCAGTACAGCGGGCGGCAGGCAGAGCGGGTGGCCAGGCGTGGGGCAGGGTGGGGGATGGGCTGTCCTGGGGTGCCTGTTCCTAGCCGTCACCTGACAGCCTGCCTCTGGGGTACATGCGGGGGCGGGGGCTCATTTAAGGCCCCACACTGGGTTTGTGCCCTGGGTGGCGGAGAGTGGTCCCTTCTATTCCCCTGGAGCCCCTTTCTTCCACTCCCGAGGTCGGTGGCAACTGGCAGAACCCTCAGCCCGCCCCACTCTGCCCGCTTCCCTCATCCTGCACTTGTGTGTGAGGGTGTGCCGGAAACTGTCGGGCCCCCCATCCCCGCTTGTCTCCCTGTATCTGCAG
Seq C2 exon
TGGGTGAATGGGGTCCATGTGTTAGAGCACGAGGGGGGCCATCTCCCCTTCGAGGCTGACATCAGCAAGCTGGTCCAGAGCGGGCCCCTGTCCTCCTGCCGCATCACCATCGCCATCAACAACACGCTGTCCCCCCACACCCTGCCGCCTGGGACCATCCTCTACAAGACGGACCCCTCCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000704:ENSBTAT00000000941:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.048 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0283713=Glyco_hydro_2_N=FE(32.3=100)
A:
NA
C2:
PF0283713=Glyco_hydro_2_N=FE(31.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGCCCTCCAGCTTCAAC
R:
ATGGAGGGGTCCGTCTTGTAG
Band lengths:
345-693
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]