BtaINT0070149 @ bosTau6
Intron Retention
Gene
ENSBTAG00000000704 | GUSB
Description
Bos taurus glucuronidase, beta (GUSB), mRNA. [Source:RefSeq mRNA;Acc:NM_001083436]
Coordinates
chr25:28172043-28172550:-
Coord C1 exon
chr25:28172369-28172550
Coord A exon
chr25:28172186-28172368
Coord C2 exon
chr25:28172043-28172185
Length
183 bp
Sequences
Splice sites
5' ss Seq
CATGTGAGC
5' ss Score
5.03
3' ss Seq
GTCCCCTTGCTCCGTCCTAGGTA
3' ss Score
8.79
Exon sequences
Seq C1 exon
TGGGTGAATGGGGTCCATGTGTTAGAGCACGAGGGGGGCCATCTCCCCTTCGAGGCTGACATCAGCAAGCTGGTCCAGAGCGGGCCCCTGTCCTCCTGCCGCATCACCATCGCCATCAACAACACGCTGTCCCCCCACACCCTGCCGCCTGGGACCATCCTCTACAAGACGGACCCCTCCAT
Seq A exon
GTGAGCAGCACCTTGCCCAGCCCCCGCTCCCACCCTCACTCCCTACCCTGCCCAGACGTCTTCCTACCAGGGGACAGGGTGGCTTCAGCAGAGGTGAGGCCCTGGCTCTTGGAGGCCAGGGAGACATGTGAGTTGAGATCGAAGGATTCAGAACAAGGCCACTGTCCCCTTGCTCCGTCCTAG
Seq C2 exon
GTACCCCAAGGGTTACTTTGTCCAGAACACAAAGTTTGACTTCTTCAACTACGCGGGACTGCATCGGTCCGTGCTCCTCTACACCACGCCTACCACCTACATTGATGACATTACCGTCACCACCGACATGGACCAAGACATTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000704:ENSBTAT00000000941:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0283713=Glyco_hydro_2_N=FE(31.7=100)
A:
NA
C2:
PF0283713=Glyco_hydro_2_N=PD(15.9=61.2),PF0070316=Glyco_hydro_2=PU(15.7=32.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATCAGCAAGCTGGTCCAGAG
R:
GTCGGTGGTGACGGTAATGTC
Band lengths:
250-433
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]