DreINT0076118 @ danRer10
Intron Retention
Gene
ENSDARG00000063126 | gusb
Description
glucuronidase, beta [Source:ZFIN;Acc:ZDB-GENE-070705-256]
Coordinates
chr5:1833970-1835488:-
Coord C1 exon
chr5:1835307-1835488
Coord A exon
chr5:1834113-1835306
Coord C2 exon
chr5:1833970-1834112
Length
1194 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAGA
5' ss Score
6.8
3' ss Seq
TTTCTGTCTTCTCCTCTTAGATA
3' ss Score
11.18
Exon sequences
Seq C1 exon
TGGGTGAACGGGGTCCAGATGACTGAACATGCGGGCGGTCATCTGCCATTCGAGGCGGACATCAGCAGCGTTCTGCGTCAGAGCTCTGGAGGTCCGTGTCGAATCACCATAGCAGTGAACAACACTTTATCTCTGGAAACACTTCCACCAGGAACCATCCAGTACATGAACGACCGCACAAG
Seq A exon
GTCAGAGGTCATACGTAAACACTGTGCTCAATTTATTTATGAGTATAAGTGTTTTTATTATGCAGAAATCACTTTTATAAGGGGTTTAAGCACAGTTGTGTGTCAGCAGTGTGTGAATATATCCAGCCTTTAATGGAAAAAAAGATTATTGTTTATAATCAGACTTGATGAAAACAGTCTGCAGAAACACTTTGATTGACATTCTCCCTTTGTACATGTCATCAGAGGGGTCAAGCCCCGCCCACTAGTGCCCATCTCTCCCTCATTAGCATAAACAGTTCTAGCAGCCACTATGCTGACACATTTGTAGCTCCGCCCTCTTTAGAAGAGAACACAATCTCATTTGAATTTAAAGCGATACAGAATGGCGGTATATGGATCAAAGTCTAAAAGAGTCCGTTTCAAAGAGATATTAAACATTATTTGTGGGCTATTTAAAGCTGAAACTTCACACACACACTCTAGCGATGCATTTATTAATATGCTTATTTTACATCGTATGAAAAAGGACATATTAGGTGTCCTTTAAATCATACTTCACCATAATTTACTCACCCTCTGGACTTTGAAGATGTAGGTGGTTTTGTTTTCTTCAGTTGAACATTTAAGAAGATTTTGAGCTTAATCTGTGCTGATTATTATAATGAAAGTCAGTGGTTACATGTTTTTGAGATTAAAAACAAACACATACAAATGAGTCCAAATGAATCCCAGTGGCTCCTGATGACACACTGGGGTCTTGTGACACAAAACAATCCGAACGTTATTTACATAGGGCTGGACAATAATTCGATATTAATATATATCATGATAGATTCTTTTTTAATAAAGGTGATATGATTTTAAAAACCCATTTCCGATGTTTTGAAATAAATTTGTATATACAGTCAAACTCAACTGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATACATACATATATATATATATTATTAGCGTGCCGACATATGGTGCAATAGCACATCAGGGCGACGCAAGTTCGAATCCCGGCTTGACGACCTTTCCCTACCCCCCCTCTCTCTCCCACTTCGTTTCCTGTCTCAATACTGTCCTATCTAATAAAGGCAAAAAGGCCAAAAATAAATCTAAAAAAAAAAGTGCATAATTAATTGGGCAAATTCATGTATTTTCTGTCTTCTCCTCTTAG
Seq C2 exon
ATATCCTTCTGGCTACTTCGTTCAGAACACTAACTTTGATTTCTTCAACTATGCTGGAATACATCGCTCCGTCCTGCTGTACACGACACCCGCGGTGCACATTGATGACATCACAGTCCAGACGACATTCTCCGATAACATCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063126:ENSDART00000091932:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0283713=Glyco_hydro_2_N=FE(31.7=100)
A:
NA
C2:
PF0283713=Glyco_hydro_2_N=PD(15.9=61.2),PF0070316=Glyco_hydro_2=PU(16.2=32.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAACGGGGTCCAGATGACTG
R:
TGTTATCGGAGAATGTCGTCTGG
Band lengths:
318-1512
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]