BtaINT0072322 @ bosTau6
Intron Retention
Gene
ENSBTAG00000013108 | HK2
Description
hexokinase 2 [Source:HGNC Symbol;Acc:HGNC:4923]
Coordinates
chr11:9734526-9736157:-
Coord C1 exon
chr11:9735924-9736157
Coord A exon
chr11:9734831-9735923
Coord C2 exon
chr11:9734526-9734830
Length
1093 bp
Sequences
Splice sites
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
3' ss Seq
ACCCCTCTCTGTGACTGCAGCTT
3' ss Score
7.44
Exon sequences
Seq C1 exon
GGAGAAGGATGGCATCCAGAAGGCCCGTGAGATCCTGGTGCGGCTGGGTCTGGACCCAACACATGAGGACTGTGTGGCCACTCATCGGGTCTGCCAGATCGTGTCCACACGCTCAGCCAGCCTGTGCGCGGCCACGCTGGCAGCCGTGCTGTGGCGCATCAAGGAGAACAAAGGCGTGGACCGGCTGCGCTCCACTGTCGGGGTCGACGGCTCAGTCTACAAGAAGCACCCCCA
Seq A exon
GTGAGTCAGCACGCGGGCCCTCTGAGAGGCCTGACTCCGCAGGAACTCAGGGTTGCACTGTGAGGGTGGTGGAGATGGGCCATGGCGTCCAGGTTCTTTGTGGAATGAAAGCTCCCCACTGGGCAAGTGGGAGCTGAAAGTGTACTGACCTCAGCATGGGTGGAGGCTTTCCTCGGAGCTCTCCTGGTTTTGGGGGGCTCTTTTTTTGTACTTTCTTAGTTCCAGTTTCTCTGGCTCACCTAGGTATTGAATTAATCCTGCCCTGCCTTCTATGTTTTGATCCCTTAGTCATAAGTGCTTAAATATTCAAATTTCAGTTGGGACTTGAAAGATATTTTTTTCTATGGTACCCACTTAGCTCATCATTTTCACCTAATAAGAACACCAGGTCAATGGCCCTGCATTAACTGAAGGGGAATGCGGAGGGTCTCAGCTCTTCCATGTCCTAGGCTCCTGGCCCAGTGCTTCAGAAACTTTAAAGTACACACAGATGACTAGAGGATCTAGTTAAGAGGCAGATTCTGGTTCAGAAGGTTTGGGTTCAGCCTGAGATCCTGTGTCTCTGATAGCACCCAGGGATGCTGCTGGCCCATGGGCCACACTTGGAGTATTAAGGCCTTGGAGATGGTGGCTCCAAGCAGCCACCACACAAATCCTTAGCTGTTCAGACACCTCTGGGGTTCTTGGCAGAAAGCAGCCAAAGAGAGGCCGAAGTCTAGAAGAGCATTTTGACGTTTCTGTTTTTGGGTCTTTCCTAGGGAGCATCTGCTGAATTGATGAAACATGAATAGTAGTTGGTGTCAGAGTCACCTGAGGCTGATAAGTTGGGCAGAGGAGCTGGGGCCTGTGAGAAGGGGGTCTTCTGTGAGTGTGTGCAGGAAATGGTGGTTTCCCAGAAGGGTCCTTTTCTTTTCACAGGGTAGTTGCAGTTAAATATTTTGAGCTTTTATGCAGTGACCCCTAACCTGGGAAAGAGTAGGGAGAGAGTGTTTAGGGTGTCATTTGGGCTGAGGTGGTGAGCTACTGCCCTAAACATTTGTACCACTGACCCTACTTGTGGGCACCCAACTCTTACCCCTCTCTGTGACTGCAG
Seq C2 exon
CTTTGCCAAGCGTCTTCACAAGACCGTGCGGCGCCTCGTGCCTGACTGTGACGTCCGCTTCCTCCGCTCTGAGGATGGCAGTGGCAAGGGGGCAGCCATGGTGACGGCAGTGGCCTATCGGCTGGCTGATCAGCACCGAGCCCGCCAGAAGACCCTGGAGTCTCTGAAGCTGAGCCGTGAGCAGCTGCTGGAGGTCAAGAGGAGGATGAAGATAGAAATGGAGCGAGGTCTGAGCAAGGAGACTCACGCCATTGCTCCTGTCAAGATGCTGCCCACCTACGTGTGTGCCACCCCTGATGGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000013108:ENSBTAT00000060994:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(32.5=100)
A:
NA
C2:
PF0372711=Hexokinase_2=PD(16.7=38.8),PF0034916=Hexokinase_1=PU(29.1=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGACCCAACACATGAGGACT
R:
AGCTTCAGAGACTCCAGGGTC
Band lengths:
355-1448
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development