MmuINT0077998 @ mm10
Intron Retention
Gene
ENSMUSG00000000628 | Hk2
Description
hexokinase 2 [Source:MGI Symbol;Acc:MGI:1315197]
Coordinates
chr6:82736530-82738520:-
Coord C1 exon
chr6:82738287-82738520
Coord A exon
chr6:82736835-82738286
Coord C2 exon
chr6:82736530-82736834
Length
1452 bp
Sequences
Splice sites
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
3' ss Seq
TCTTTCTCTGTGTGTCACAGTTT
3' ss Score
9.15
Exon sequences
Seq C1 exon
CGATAAGGACGGAATTCAGAAGGCCTACCAGATCCTGGTGCGCCTGGGTCTGAGCCCGCTGCAGGAGGACTGTGTGGCCACGCACCGAATCTGCCAGATTGTGTCCACGCGCTCAGCCAGCCTGTGTGCAGCCACCCTGGCCGCGGTGCTGTGGCGGATCAAAGAGAACAAGGGCGAGGAGCGACTCCGCTCCACCATCGGCGTGGATGGCTCTGTCTACAAGAAACATCCCCA
Seq A exon
GTGAGTGAGCAAGCCGGGGCTGGGAACAGAAGGGTCCCCTGGAGACTCCATGAGGCAGCTCTGTATTGCTTTCCAGACAGCATAAGTGTTCTCCAGTCACTTGGTATGAGTGGGATTGCTTTCTGAAGGCAGCTGATGGCTTGCCCTCTGTTTAGAGCTCCTGGTTGCTGAGTCCTTGGCTTGCTGCCTCCTCTTCCGCTCTGTTTTGAGACAGGCTTGTCCTCTAGTCCCTCCCAGGCTGGCCTTAGACTCAATCTGTGCCCATGGCGAACTTAAATTCTGATCCTCTTGTCTGTCCCCGCCTCCCAAGTTCTAGGATCACAGGTGTGTACCACCTTTCTGCAGAGTTTGCCATTGAATGCTCTGTGCATGGCAGGCAAGCACGTCCTCACAACCCAACATCATCCCGGGCCACCTCCCTCTTTTTATGAGTTACTGATTCATTCCTTGTTCTTCCATTACTCTGACTGGTTCATGTGGGTACCATGCCCTCTGTTAGTGGCCATCCAACCTTGAAGACGCCAGCCTCTCCTGATTTTAGTGTCGGATTGTTCCCGCCCTGTCATGTCCCTTTGTTCGTCTATTCCAAACTGTTCATATCTCACCTGAGATCCAGAAGAGGTCTTTTGTGTTCCCGTATTTTTCTGTCATTTTCTCAGTGTGAGTGCCAGGTCAGGGCACCTTTAACTGAGCTGGTGGCTGCCATCTGGCTCTGAGATCCATCTTCAGCATTCTAGCCCAGGGCTGTAGGAACATGGATAACCTGGGAATGTGGTTAAGAGGCCGGTTCTGGCTCAGTGACTGGGTGAGGCTGACTTTTACAGTGACTGGCCTGCCGCGAGGCTGCTTGCTATCTGTGTGGGTGTGGGTGGTCTGGGAGAGGAGCCTTGGAATTGCTGGGCTGGGCTGTGCTGTCCGCAGCAGTCTGCGCTCCGTGGAAGCTGCAGGGGGCCGCCACCCAAGAGCTTCTCCCTAATTAAACATTCTCACTGTCTCCAGTTGTTCAGATGCTTCTGAGGCCATTGGCAGGAACTGGAAAGAGAAAGGCCAGAGTCCAGAGAAAGATACTCGTAGGCTTTTGTTGTCATTGTTGAGTGTTTTCCTGGGGGCCATCAGATGAAGTGAGCCTGGATCTCCAGGGGCATCAGAAACATTTGATTCCCGTCGTCTGCCCTGGCAGGGGCTGGCTCCATCTGCTAAGCAGAGGAGCTGAGGCTTGTTCCATGAGAAAGAGTGGGTCTGTGTGACTGCGTTCACGGGGGAAGCCAGCTAGGGCAGCCATGATTGAATAGCTGGGCCTTTATATTAGTCACCCTAAGATGGGGGTGGGGGAGGGATGGAGAGAGGAGATACAGACACCACTTGCTTCTGCAGGGGCCATGGTCAGCTCTGCGGCCATTTGTACCACAGACTGTAACCATGGACACCCGTCTCTTTCTCTGTGTGTCACAG
Seq C2 exon
TTTTGCCAAGCGTCTCCATAAGGCAGTGAGGAGGCTGGTGCCCGATTGTGATGTCCGCTTCCTCCGCTCTGAGGATGGCAGCGGCAAGGGGGCGGCTATGGTGACGGCGGTGGCTTACCGTCTGGCTGACCAACACCGGGCCCGCCAGAAGACCCTGGAGTCTCTGAAGCTGAGCCATGAACAGCTGCTGGAGGTTAAGAGAAGGATGAAGGTGGAAATGGAGCAGGGTCTGAGCAAGGAGACGCATGAGGCCGCCCCTGTGAAGATGTTGCCCACTTATGTGTGTGCCACTCCAGACGGTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000000628:ENSMUST00000000642:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.097
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(32.5=100)
A:
NA
C2:
PF0372711=Hexokinase_2=PD(16.7=38.8),PF0034916=Hexokinase_1=PU(29.1=58.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACCGAATCTGCCAGATTGT
R:
AACCTCCAGCAGCTGTTCATG
Band lengths:
349-1801
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types