BtaINT0089051 @ bosTau6
Intron Retention
Gene
ENSBTAG00000010576 | MAP2K3
Description
Bos taurus mitogen-activated protein kinase kinase 3 (MAP2K3), mRNA. [Source:RefSeq mRNA;Acc:NM_001083693]
Coordinates
chr19:35872372-35874289:+
Coord C1 exon
chr19:35872372-35872449
Coord A exon
chr19:35872450-35874149
Coord C2 exon
chr19:35874150-35874289
Length
1700 bp
Sequences
Splice sites
5' ss Seq
ATGGTACGG
5' ss Score
8.21
3' ss Seq
CAGTGCTATCTCCACTGCAGATC
3' ss Score
7.76
Exon sequences
Seq C1 exon
CCTGAGAGAATCAACCCAGAACTGAACCAGAAAGGCTACAATGTCAAGTCCGATGTCTGGAGCCTCGGCATCACCATG
Seq A exon
GTACGGCCTGGGGCACCCTTGCTGGTCAGGTGGAGCACAGGTGGGGGCTGTGCCAGGTCTGTGGGCTTCTGAGGGCCAGATAGTCTAGCCTGGACAGAAGGGTTGGTTGGCTTCACAATGTGAGAAGAGACAGTGCCATCAAAGATTATGGTGTCCTCAGTGGCGTGTCCGCAGATGCCTCACGCCCTCCCTGGGTTAGGGCAACGTTATGCCGCTCTCGTCGGTGATGACTTCCTGTTGGACTTCCTCAGGGCTCGTGGTGCTCACTGAGAATGCACAGCCAGCCCCAGACCAACTGGCTCCCTGTGCACGAGGGCTTCACAACAGTGGCGAGGCTCAGTGACCTGCCCTACTCATTTTTGCTGCTTCTCCACGTTTACTCCCACAAATAATGCCATACTATGTGTCTCTATGCCCAGAGGCTGCTTCTGTTTTCATTTTTTGAGCTCTTTTAGAAAAAATTACCAGGAGAGAAATGACTAGGTCCAAGGTTTTTGTCCTTAAGCTGTTTTCGTCCTTAAGTTCTGGTGGCCACTGCATCCTTCTCTGCAGCCCAGGCAGGGTCCTCACTCCTGTGTGCCGGAGTCAGTGGAAACTATAGGGAAGAAAGGCTGTCTTGGCCCTATGAAAATCCAGGGTCATAGCCATTCCCGCCACACTGTGTCACCTCCATCTGCGGGCCTGTTTGTGTCTTGGCTGGAGGGGAGCCTAGAGCCTGATGAGAAGGTGCTTTTTGAGCACAACTGGTGACCTGTTTGGGTGCGTCTGCTTGAGTTGAGGTGTCTCCTGTTTATTATGGTGATCGCTTGTCATGGAGGTGGACCTGAGTTTTCATGTCAGCAGTCAAAATTGGAAGCAGAGTTTGTTGTGAGCCAGCCATTCTTTCCTACAAATGACTCCTGCAGGCGAGGCATGGGGCACAGGGAGATCTGGGTCTAGTAGAAGCGATTGAGGCCATGGCAGCCTTGTGTCCAGGGAGTCGAGGAGAAACAGTGGCAGGATCAGAGTAGAAAGGCTTCCTGGAGGGGGAAGCATTGGAGGTGGGGTTCCCAGGCGTTGGTGGCTGGGTGGTGGGGGCTTTGTGGAGGAGAAAGGGCTGCAGACTTCATTTCCTAATGGGGCCTGGTAGATTGCTGCACAGCCCAGAAGATGAACCTCTCAGTCTAACATTCCTCCTGCAAGCTCATGAGAATCATTTCATTTCCCGCTTGCCTTTTCAGTAATAACAACAGCCCACATAAACGGCAGCAGACGCTAAGGTGTTGCTGAGACATTAGGGAGGAGGGAGTGGTTTGTGGGGAACTTCGAAGCTGATCGCTCTCCTAAGGCGCTTTGCTGACAGCGGCTTTATGGGGAGCACCCCAGGCTGCCAGCCCTCTGATTTTTCCAGGTTAGCTGGAACCTGGGTTTTAATGCAAAACCTTCTGAATTCAGATTGTTTGAAAACCTGATAGCCAAGCCAGTCAAAACTCAGGGTTGAATTTGTACCATGAGCTATATAGTCTTTGCTGGCTGGGACAGAAGAACTAACTCCTCTGTCCCTTGGCTTTCATCTTTGGACTGATGTCACCTGTCAGCCCCTTACCCTGCCTCACAGTCCAGCTCTGGCTGCTGGACTAGGACAGGCAGGATCTTTGGAGGCTTTGCTTCCGTGTGGGAGCGTGGGTGTCTCTGACCCAGCAGTGCTATCTCCACTGCAG
Seq C2 exon
ATCGAGATGGCCATTCTGCGATTTCCTTACGAGTCCTGGGGGACCCCCTTCCAGCAGCTGAAGCAGGTGGTAGAGGAGCCGTCCCCCCAGCTCCCAGCTGACCGTTTCTCCCCTGAGTTTGTGGACTTTACTGCACAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000010576:ENSBTAT00000013983:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.085
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(9.5=100)
A:
NA
C2:
PF0006920=Pkinase=FE(17.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGAGAGAATCAACCCAGAACTG
R:
CACTGTGCAGTAAAGTCCACA
Band lengths:
218-1918
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]