GgaINT0015627 @ galGal4
Intron Retention
Gene
ENSGALG00000004735 | MAP2K3
Description
dual specificity mitogen-activated protein kinase kinase 3 [Source:RefSeq peptide;Acc:NP_001012805]
Coordinates
chr14:4491242-4492716:-
Coord C1 exon
chr14:4492639-4492716
Coord A exon
chr14:4491382-4492638
Coord C2 exon
chr14:4491242-4491381
Length
1257 bp
Sequences
Splice sites
5' ss Seq
ATGGTAAGG
5' ss Score
9.33
3' ss Seq
CACTGTGTCTCTGTCCCCAGATC
3' ss Score
12.58
Exon sequences
Seq C1 exon
CCAGAAAGAATAAATCCTGAGCTGAACCAGAAGGGCTACAACGTGAAGTCGGATGTCTGGAGTCTGGGGATCACAATG
Seq A exon
GTAAGGCAGAGGGCATGTGCCAGCACTGTGATGCTGGGGTCTGTGCTGGGGGCTCCAACCTTGAGGTGGTGTGTGTGGTTAGCAGCAGCCCTGCCTGTGGGGCAGCTCACAGCAGCCAACAGGAAGGGGAAATGCTTTCAGTTCCAAAATTGCTGCTGCTCAGGATGGTTCCTCTGCCTCCTGCAAGAAATGGATCTTTAAGAAAGCAGCTGAAATCTTCTGGCACAGGAGGACAAAGGAAATCCCAGCTGCTGGCTCAAAGGGAATGCTGCCTGGGAACACTTCTGCTTTCCAGGAGGCTGCATCTCTTGCTTAGACTATTTCCCTGAAGTTTTCCTGCACCATTTTAGCCCTGACCTTTGTTTTGGACACTGTCTCCTTGCATCTGTGTGTGCTGCACAGATCTCCGACAAATACTCCCTCTTGGTATCCCTGTGCTGAGCAGAGCTGGGCTGGCTGCAAAAGCAATCAGCTCCAGCTCTGCTCTGAGGTGCAGGAAGCTGTGGGATGGGAGATTGTGGCAATGACGAAATCTCTGCACTCTTCCAGCAGTGGATGAGAGAGGGGACCTTGCTGAAAGCCTGCCAGCCTGCAGCTGGATGGAGCCCCAACCTCATTCCCTCTAATATCTTCCTCCTGGGCTGGCTTCCTGTTTTGTTTTTTATTTCTGGGGGGTGGAGGTTTGATTGAAGACCAGCCTCATTTAGGGCTGTTGAGCTGCAGGACTGATCCTAGCTTTATTCATTTCTCACATCCCATCCCTTGGCCCAAGAGCTGCGGTGGGCTTTTTCCTGCTGGCATGGATGATGGAGCAGGGATAGCTGCAGCAGTTTGGCAGAGATCATCTTAAGTTATGTTCTACAGCTTGCTTTCTCTTGAGCTCATGGATCTCTTTCTGCCTAGCTCATACAGCCTGCAGCCCAGAGGGCAGGTTATGGTGAGAGGAGCACTCCAGAAGCCAGTGCAGAAGTAGCATCTACGCTGCTTTATCTTTTTAAATAACTCTCTTTCCCATATATTTGTCTGCTTCCTGTAAATGCATTAATTTCCTGAGCAGGCCTCAAAAGGAAGTATTTTTCCCTCTAGGTCTTTGTATTTAAAAAAAAAAAAGAAAAGGGGAGGGGGGAGGGGAGAGGAAGGTAGTATGACCCAAACTGAAAATGGTTTGTTAGGAGGGAGGCTTTTCATCTTGATTCTGCACTTGTGCAGCCTCTGATGTGGGGCAGCCCCAGTGTCTCACTGTGTCTCTGTCCCCAG
Seq C2 exon
ATCGAGTTGGCCATCCTCCGCTTCCCCTATGAGTCCTGGGGCACCCCGTTCCAGCAGCTCAAGCAGGTGGTGGAGGAGCCCTCACCCCAGCTGCCCCCCGAGCGCTTCTCCAAGGAGTTCGTGGACTTCACGGCGCAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000004735:ENSGALT00000007540:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.085
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(10.6=100)
A:
NA
C2:
PF0006920=Pkinase=FE(19.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGAAAGAATAAATCCTGAGCTGA
R:
CTGCGCCGTGAAGTCCAC
Band lengths:
216-1473
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]