BtaINT0097065 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018204 | MYH1
Description
Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), mRNA. [Source:RefSeq mRNA;Acc:NM_174117]
Coordinates
chr19:30123150-30123637:-
Coord C1 exon
chr19:30123501-30123637
Coord A exon
chr19:30123406-30123500
Coord C2 exon
chr19:30123150-30123405
Length
95 bp
Sequences
Splice sites
5' ss Seq
GAGGTACTC
5' ss Score
6.43
3' ss Seq
CATTTCTGCTCCTATCACAGAGA
3' ss Score
6.73
Exon sequences
Seq C1 exon
GTCTTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGATGAAAAGCTAGCCCAGCTGATTACCCGAACCCAGGCCAGGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAGATGGTGGAGAGGAG
Seq A exon
GTACTCTATGGGAAACTTTCTAACCCCATCAGCCTATGCCCTGTAATTCCGTTTCCTATTATACCTGACTTTGAACATTTCTGCTCCTATCACAG
Seq C2 exon
AGAGTCCATCTTCTGCATCCAGTACAATGTCCGTGCTTTCATGAACGTCAAGCACTGGCCTTGGATGAAGCTGTATTTCAAGATCAAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAGGAAATGGCCAACATGAAGGAAGAGTTTGAGAAGACCAAAGAAGAGCTGGCTAAGTCAGAAGCAAAAAGGAAAGAACTTGAAGAGAAAATGGTGACTCTGACACAAGAGAAGAATGACCTGCAGCTCCAGGTTCAGTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018204:ENSBTAT00000009327:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.256
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=6.5),PF0061222=IQ=WD(100=43.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=PU(27.4=62.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCTTCTGGGGCTCCTAGAGG
R:
ACGTTCATGAAAGCACGGACA
Band lengths:
169-264
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development