DreINT0100046 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40753292-40753755:-
Coord C1 exon
chr6:40753619-40753755
Coord A exon
chr6:40753548-40753618
Coord C2 exon
chr6:40753292-40753547
Length
71 bp
Sequences
Splice sites
5' ss Seq
GAGGTATGG
5' ss Score
8.98
3' ss Seq
GACCATTTCTTCTGTTACAGAGA
3' ss Score
8.67
Exon sequences
Seq C1 exon
GTGTTCTTCAAAGCTGGTCTGCTGGGTACTCTTGAGGAAATGCGAGATGAGAAACTTGTTATCCTTGTAACCATGACTCAAGCAGTCTGCAGAGGCTACGTAATGAGGAAAGAGTTTGTCAAAATGATGGCGAGGAG
Seq A exon
GTATGGATTTTGCTTAAAGAAATATCCAGTGCACATTCACACACAGCATCTGACCATTTCTTCTGTTACAG
Seq C2 exon
AGAGTCCATCTACTCCATCCAGTACAACATCCGCTCATTCATGAATGTGAAACACTGGCCATGGATGAAGCTGTATTTTAAGATCAAGCCCCTGCTGAAGAGTGCAGAGACTGAGAAGGAAATGGCAGCCATGAAAGAAAACTTTGAAAAGATGAAAGAGGATCTTGCCAAAGCCCTTGCAAAGAAGAAGGAGCTGGAGGAGAAGATGGTCACCCTGCTGCAGGAGAAAAATGACCTGCAGTTACAAGTGGCATCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.047
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=6.5),PF0061222=IQ=WD(100=41.3)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTCAAAGCTGGTCTGCTGG
R:
GCGGATGTTGTACTGGATGGA
Band lengths:
167-238
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]