BtaINT0097069 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018204 | MYH1
Description
Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), mRNA. [Source:RefSeq mRNA;Acc:NM_174117]
Coordinates
chr19:30117162-30119172:-
Coord C1 exon
chr19:30119046-30119172
Coord A exon
chr19:30117281-30119045
Coord C2 exon
chr19:30117162-30117280
Length
1765 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGC
5' ss Score
8.07
3' ss Seq
CTGGTTCTTGGTCTTTATAGGTG
3' ss Score
9.56
Exon sequences
Seq C1 exon
GGAAACCTCGAAAAGATGTGCCGCGCTCTAGAGGATCAGCTGAGTGAACTTAAGACAAAAGAGGATGAGCAGCAGCGGCTGATCAATGACCTAACGACTCAGAGAGCGCGTCTGCAGACAGAATCAG
Seq A exon
GTGGGCATCTACCAGTCCTGACTTTGAATGACCAGTTTACCTTGGATTCTCAACTGGCTGATAAACCCTGCACTGAGTTACTTGAGCTCATGGGAGGTGCTGCTAATATGAACACCTTAGCTACCACCGGCTTGGACATGTATTCATCAATGCAATGGACATGAACTTGGACAAACTTTGGGAGATTGTGAGGGACAGGGAGGCCTGGCATGCTGCAATCCACAGGGTCACAAAGAGTCAGACATAACTGAACAACAACAGTATTTACTTTCACTTTTCAAAATGCTTCCACACATTAATTACTTTTTTACCCTCATTAGTCTGTATAATGTTATCCCCATTTTAATGATGTTAGATTACGGTTGCAGAGAAATGACCTGTCCAAGAATGCTTACTCCAGTCTTTTCCCTGAAGTCTTGACAATTTCTGGTAACACCACATTTAAGTAGGGAACTATTCAGGACCACGATCTAACCAGGAGCATGCAAGTGAGATTCAAAGAGTTCTGATTATCCAAACTGGAGAGTCTCTGCATTTACTCCTCCCCGTATGTACCAACTTGTCCAATTTTCTACCAAAGTTCTGCCTCCCCAAGTGAGAGGTGGAAATTGCTTTACGGGTCTTTTGAACCAGTAAAGCAATGGGTGGATCAAATTGTTGGAGAACCACTTTTACTCTTTCATATTCATTCAAATATTGTTCTTTCTAGAACAAAACTCAAGTAAGGCTTGTTATTACTGTTATACAGGTGGTAAGTACATTTCATGAAAAGAAAAAATTGTCTTCTCAAAGAATCAAAATGCCCCTTGTAGTTTCTTTTCCTCATTACATGTTTGTTCCTAGTAATCCAGTGGTATGAAAAAAAATTGGAAGTTGTGATTCTTATTAAATGTTAAACAGGTAAGGCTTCCAGGTTATCTGTTCAAAAATGGTGCTGAAAGGGTTTTCCCTCTTTTTAACATAATACATTTGACCCTTGAACAACATGGGTTTGAAGAGTGTGGGTCCACTTATACGTGAATTATTTCCAATAAAAATGCACTATAATACTAGATTATCCACAGTTGGTTGAGTCCATGGGTGTGGACAGAGAGGGCTGACTGTAAAGTTATATCTGGGTTTCTAACTGCTGAAAGGGTTGAAATCCCTTGGTTGTTCAAGGGTCAACTGTATAGTATATGTTCAAATTATGGTGCTTAAAGAATGGAAGACAAACTTAAATGACACTTAGTTTAGGAACTGATTTGTTATCCACAGACCAACTCATTCTTGGGGTGGAAGGACGCTTGACTGTCTCGATATAAAAATTCTTCCAGGATTTCTCTTTGAACATCAGTTCAAAGGGTGCTGCTCCAAGTCTCCTATTTTCCACAGAATAAATTCTGTTTCAGTCAAGTTTCAGTGGAAGGTAAACTAGTACAACACCATTGCCATAGACAAAAAACACTGGAATGCTTTTTGTTAGTCTGATTTATATTTCTTTTCACATATTTCTTCATCTATTTCTACTGTGATCAAACAATTTTAAATATCTCCTGAAATCTTTTCTGTTGTTCAGAAGAGTGTAATCTGCCTTAAAGTCTTCCCTACTTCTCATTTTCACATGGATGTGCTCACCAGGGTTGACGTAGAAATTCGTGGTTTGCTGTAATCCACACTCTGCATCATGTTTCAACCCCAGCTCCCTTTCCCCTGCTCCAGTTTGTATCTTGGGAGTTTTAATTTCCAAAGTGAGGTTGAGTTTTCTGGTTCTTGGTCTTTATAG
Seq C2 exon
GTGAATTCTCACGCCAGCTAGATGAAAAGGATGCACTCGTTTCTCAGCTCTCAAGGGGCAAACAAGCATTCACACAACAGATTGAAGAACTGAAAAGGCAGCTGGAAGAGGAGATAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018204:ENSBTAT00000009327:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.767 A=NA C2=0.225
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.9=100),PF0003816=Filament=PU(25.8=55.8)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=FE(41.9=100),PF0003816=Filament=PU(8.9=62.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAACCTCGAAAAGATGTGCC
R:
CTTTATCTCCTCTTCCAGCTGCC
Band lengths:
246-2011
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development