DreINT0100053 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40751148-40751683:-
Coord C1 exon
chr6:40751557-40751683
Coord A exon
chr6:40751267-40751556
Coord C2 exon
chr6:40751148-40751266
Length
290 bp
Sequences
Splice sites
5' ss Seq
ACGGTAAGT
5' ss Score
11.81
3' ss Seq
AATCATTAAATTCACAATAGGTG
3' ss Score
5.33
Exon sequences
Seq C1 exon
ACAAATCTTGAAAAGATGTGTCGTACACTGGAAGATCAGCTGAGTGAGTTTAAGTCGAAACATGACGAACATGTTCGCCACATCAATGACCTAAGTGCTCAAAAGGCAAGGCTTCAGACTGAAAACG
Seq A exon
GTAAGTAGCTTTACTATACGCCAGGGGTGCCCAAACTTGATCCTGGAGGGTCGGTATCCTGCAAAGTTCAGCTCCAATCCCAAGTAGACACAGCTGAACCAGCTAATCAAGCTCTTACTAGTCATAGTGAAAACTTCCAGGAAAGCGTGTTGAAGCAATTGGAGCTAAACTCTGCAGGACACCGGCGCTCCATAACCATGTTTGGAAACCCCTGGTTTACAGGAACGATATTAAAAATGACATTCTTCATAAACAGCATATACAATACTAAATCATTAAATTCACAATAG
Seq C2 exon
GTGAAATGGGTCGTCAACTAGAGGAAAAAGAGTCTTTAGTTTCTCAGCTGACTCGAAGCAAACAGGCTTACACTCAACAGATTGAGGAGCTGAAGAGGCAAATTGAGGAAGAAGTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:28
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.860 A=NA C2=0.775
Domain overlap (PFAM):
C1:
PF0003816=Filament=FE(14.5=100),PF0157614=Myosin_tail_1=FE(4.9=100),PF131661=AAA_13=FE(19.4=100),PF0003816=Filament=PU(1.9=14.0)
A:
NA
C2:
PF0003816=Filament=PD(6.2=45.0),PF0157614=Myosin_tail_1=FE(4.5=100),PF131661=AAA_13=FE(18.0=100),PF0003816=Filament=FE(12.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTGAAAAGATGTGTCGTACACTGG
R:
TGACTTCTTCCTCAATTTGCCTCT
Band lengths:
239-529
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]