BtaINT0097185 @ bosTau6
Intron Retention
Gene
ENSBTAG00000002580 | MYH14
Description
myosin, heavy chain 14, non-muscle [Source:HGNC Symbol;Acc:HGNC:23212]
Coordinates
chr18:56909494-56911379:+
Coord C1 exon
chr18:56909494-56909616
Coord A exon
chr18:56909617-56911191
Coord C2 exon
chr18:56911192-56911379
Length
1575 bp
Sequences
Splice sites
5' ss Seq
CGGGTGAGA
5' ss Score
7.62
3' ss Seq
CTTTATTCTTTCTCCCACAGTGG
3' ss Score
10.35
Exon sequences
Seq C1 exon
AACACGGGGGCTTGCAGCAGTTCTCTTTCCTTGGCTCCTTCCCACCGTCGCCCCCAGGATCTTCAGGGAGGCACAGCTCTGCCATTTCTCCATCAGGGGGTGGGTGTTGCTGTGCATCAACGG
Seq A exon
GTGAGATTTCCTGCACGAGGAGGGTGGGCCCTTTGGACCGGGAACCTGTTTTGAATGTCTATCCCCTTTCCTCTCCATCCAGAGATCTCCCCCTCGTCTTTTCCCCCCTCTTGTCAGCTCCCTCAGCAGTTTCCAGATTCTTTGTAGCAGCACAAGGCCTTCTTCGAACTAAACCACATGTAAAAATACAAACAATTATGACAGTATTAAGCTCATTTGGGGTTTTTTGTAATATTGTACAAAAACCCGGACGAATCTTTTGGCCACCCCTCTATCAGCTGGCATTAACCGAGCATATAGTGTGTGCTGTGTGCTGCGCAGAGCCCTTTACTATTATCAACACATGGAATCTTCCAGACACCCAATCAGGTGTTAGAGCTCTTACCCTGTTATAGATGAGGACATGGAAGCCCAGAAAGGTTAAGTCATCTGTCTGAGGTTACGCAGCCAGTAAGGGACCGAGCTGGGATTCGAACCCAGAGCCTCCCCTCTCATCTGTCCTTCCGATGATAACTAGGAGCATGGCGGTGGGGGCTGGGAGACTTAGTCCTGGGGAGGGAAAATATCATATCTGAACCCTCACTGCTCACCAAGCTCTGCTTTCCGAGAGAATTATCTATTGATTCCATAACCCTCACGACTGGGGATTGCAGGCCATCAGATTCTGCCCCAGCTGCAGGTCCTGGAACCGCACGTGATTTAATACATTGAATTTGTGACAAAGGAGAGGCTATTGCTCTCTAGCTGAAGTGACAGCATCAAATAATAAAACAAAGTTACAGAACCTTCCTGGTGGCCCCGTGGCGAGGGTGCCATGCTTCCAGTTCAAGGGCCCTGGGTTCGATCTCTGCTCAGGAAACTAGATCCCACGTGTGACAAATAAGACCCAGCCAGCCAAATAAATAAATAAATATGTATTTTTAAAAGACAAAGTTACCATCAGGCCTTTCTGGGAAAATGGTGACTTTGGAGGTAGTTGGTATTATTTGTTTTTTCTTTTCTTTTTTAAAATTTCTTTTTTATTTTTGGCCTTATTTTAAATATTACCTTCTTTCAAATATCATAATAAAGCCACATTTCACTGCATTTTTAACATGAGACTGTTTTAAGTTCCTAAAAATGCACCTGTGGTTATCACCCTTTTCTTTCTCTGTGCCTCAGTTTCCTCATCTGTCAAATGGGGGTGACTCTTATAGGCTTATAAATAAGCCAATGTATGTCAAGTGCTTTGAGCAGAACCGGGCAGAGAATTGGTGCGAAGGAAGCATTTGGTATCATCATCATCATTTGCTGATAAGGAAAATGGAGAAGAAGGGTCAGGGTTTGAGAGGATTCACACCACTTTGGTTCAGATCCTGGATCTTCAGATCCTAAGTTGTGTGGCCTTGGGCACAGTTGTCCAGCTTTCTGAGCCTCAGTTTGCTCGTCAGCCCAATGAGCTGATAATACCTGCCTTGCTGGGCTATGAGAGATGGCTCATATACAAAGTATCAGGTCTTATAAATAATGAGATCCTTGACTTCGGCCAACTCAGTCCCCTAAAACTGACCCCTGACTTTATTCTTTCTCCCACAG
Seq C2 exon
TGGAGGGCATCGTGGGGCTGGAGCAAGTGAGCAGCCTTGGGGATGGCCCGCCTGGTGGCCGCCCGCGCCGGGGCATGTTCCGGACAGTGGGACAACTCTACAAGGAGTCCCTGAGCCGCCTCATGGCCACACTCAGCAACACCAACCCCAGCTTCGTCCGCTGCATCATCCCCAACCATGAGAAGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000002580:ENSBTAT00000003343:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.452 A=NA C2=0.254
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.7=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(8.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTGCAGCAGTTCTCTTTCC
R:
CCTCTTCTCATGGTTGGGGATG
Band lengths:
302-1877
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development