Special

MmuINT0103338 @ mm9

Intron Retention

Gene
ENSMUSG00000030739 | Myh14
Description
myosin, heavy polypeptide 14 [Source:MGI Symbol;Acc:MGI:1919210]
Coordinates
chr7:51889729-51893249:-
Coord C1 exon
chr7:51893135-51893249
Coord A exon
chr7:51889917-51893134
Coord C2 exon
chr7:51889729-51889916
Length
3218 bp
Sequences
Splice sites
5' ss Seq
ATGGTAAGG
5' ss Score
9.33
3' ss Seq
ATCTCTTATGCCCTCCACAGTGG
3' ss Score
7.97
Exon sequences
Seq C1 exon
GTTGACTACAAAGCCAGTGAGTGGCTGATGAAGAACATGGACCCACTGAATGACAATGTGGCCGCCTTGCTTCACCAGAGCACGGATCGTCTCACAGCTGAGATCTGGAAGGATG
Seq A exon
GTAAGGTCCCTCTACCCAGGGTCTGTCCCCAGATGCCATGTCCCTTCCTACCCCCAGTGGGAGCAGGAGCCAGAGGCTAGAGGGGGAGGGAGGAATGGTGGGTATGGATGAGTGGGTAGGTGGGTGGGCCAGTGGATGGATGGATGGATGGATAGATAGATGGATGGATGGATAGACATATGGATGGACTGATTGGTGGATGATGGATGGGCAAGTGTATAGATAGGTGGATGAGGGGGTTGGTAAGTGAGTGGGTGAGTTGGCGGATGGACAGGTTAGCAGATGTATAAATGGACAGGTAGAAGAATGGAAGGCTGGATGTGTTGAAGAGAGGGTGGATGGGTGGGTGGGCGCATGGATGGTTGGATTGGTGAGTGAAAGGATGTGTTGGTAGATGGGTAAGCAGGCAGATGTAGATGGATGGGTGGAAAGCTAGGTGGGTTGAGGAGTGGGTTGGTGTATTGATGTGTTGTTATCTAAACTAACATCTTACACAAGGATAAGAAATAATACAGGCCTACCCATGGGCATATTAGTGTCAATGATCGTTTTTAGTAATAGTCTCCTATGCAATCAGACCAGAAAAAATAAGTGCAGCCATCCCTTGGGATGCACAGGACACTAGGCCACTCAAAGACTTACCTAGATACCCTAGTATTCACATTTGACACACACCCCTTCCTGTATACGTTTTCCTCTCTGTCCCTATAGGCTGAATCAGCCCTCATCCTATTTATTTAACAAGATGCAAATGATATGTAAATAGCAGTTACACTACTACACAGACTATGGCAGGAAAAGCCTGTGTTGTGTTCAGTAGAGACTCTTTTCCTCGGAATAGTTTTAATTAGTTGTTCAACCGCAAGCGCTGAGTCTGAATATATGGAAAGCTGATTTTATATAGATATTTTTAGCCAAGCATGGTAGTGCAAGCCTTTAAATCCCAGCACTCGGAGGCGGGTGGGGCGGGGGAGGCATCTCTGTGAGTTTGAGCGCAACCTGTTTTACATTATGAGCTCTAAGGAAGCCAGGGCTACAGAGAGGCCCCGACTCAAACAAACAAACAAACAAACAAACAAACAAACAAGTTGAGCCATCAGAGAACCCGAGTTCAGGATCGACACCCATGTCAGGTGGCTAATAAGCACCTACAACCACTCTTAACTCCAGGGGCTCCAACACCTCTGTCCTCCATGGGTACCTCAATACACACACACAGAGACACAAAATTACAAATAATCAGAATAAATCCTTTCTAAAGCTTGGTGTAGTTCATGGGCACTGTGGGCACATGACCCCTTACCCATCTGTCCCAGCCCCTGTGTGCCTGGCTCTGAGCGACAGTCCTGATCTTCTGGAGCTGATGCTAAACCAATCTCCCCCCTGAGGAGTCACTAACAAGGCTGGCACAAAGGGCAGGAAGGGGAAGAGTAGGGACTTGGTCCAGTTTACATCCATCTCGCGCTTGGCCACCTTGATCCCAGAATTGGACATGCAGCCAGGGGTCAGGGGCGGGCAAATGAGACAGTCCTTGTCTCAGTTAGGGTTTTACTGCTGTGAACAGACACCATGACCAAGGCAACTCTTATAAGGACAACATTTAATTGGGGCTGGCTTACAGGTTCAGAGATTCAGTCTATTATCATCAAGGCAGGAACATGCCAGCATCCAGGCAGGCATGGTGCAGGAGGAGCTGAGAGTTCTACATCTTCATCTGAAGGCTGCTAGCAGACTACTGGCTTCCAGGCAGCTAGGATGAGGGTCTTATAGTCCACATCCACAGTGACACACCGATTCCAACAGGGCCACACCTTCTAATAGCACCACTCCCTGGGCCAAGCATGTACAAACCATAACAGCCCCCAACAGGTGGGGTGGATATGTCAAGTTAGTTGCTGCACTTCTGGGCTTCCAGGGCAAAAAGGACTAGCTAAGACAACAGTTGCAGACTTGGGTTCTAAGGTGTTCTGTCATTTTCTTTGGTACTGACTCCTCACAGATGCTCTCGGCACCGATATTAGGGTGAACAGGACTTGATTAGGTCACAGGCAACAGCTGAGTCCTGCCTGCTTGCTCTGTGCTGACTAATGCTCTCTCTCTCTCTCTTCCTCTCACCTTTCCCTCTCCCATCTCCACACATCTCTCCCACCACTGCCATCTTCCCCACCCCTCATCTGCCCATCCCCAGAACAAGGGGGTCTCCAACAGTTCACTTTACTTGGCTCCTTCCCATCGCCGTCCCCTGGACCTGCAGGGAGACTCGGCTCTGGCGCTTCTCCTCCAGGGGTTGGGTCTCTCTGTGCACCCACGGGTGAGGTTGCAGAATGCAGTTGGGAGGGACTGTGGGGAGGGGTCCTGGGTGGAAATGCTGGTTCCCCTCCTCTCTGCATGAGGTCTCTCCCTCCTTGCTGAGCCCTTGGTGGTTCTAGAACCTTCTAGCTTGACCACTATTAAAAATACAGAGAAGGGGCCAGCAAGATGGGTCAGCACATACAGGGGTGTGTGCCGCCAAGCCTGACATTCTGAGGTCCATCTCCCAGAACCCACATGGAGGGAGGAGAGAACTAATTCCCACAAACTGTCTTCCACACATGCCTTACACGCACGTGCACACTCACATGAACACACACACAGTGTGATAACATTTTAAGAAAGACATTTCCTGAGCACCTAATGTGCAAACAAGGAAACAAGTACAGAGAGGTTATGTCGCTTGCTCAAGGTCACACAGTCAGTAAGTGGTAGAGTCGGGATTTGAACCCAGAACCTTTCCAGCTCTTTCTTATGCTGTAGAAACCTAATCAATGAGATGTGTCATTTTGAAAATGACTGACAGCATAGGGGCTCCAGAGAGACTTGAAATTGGGGACTCCCAGCAGTGGTAGGGCACATGAGGACACATTACCAGGTTCTGGCCCTGGGTCTTTGCTTTCTGAGCTATGTGACCTTGAGGCCAGTTGCTGCATTTCTGACTCTCTGTCTGCCTGTCAACTGCAACCCCCAGCCCCCAGGCCAAGTAGCAGTGCTCTGTGTGACATGCCAGGTCCTATAAACAGCAAGATACTCCACCTCAGCTAACTCCATTCCTTAAAGATGATTCTAAGGAGCTGGGTGTGGTGGCTCCAGCCCAGCACTCTGGAGTCTGAGGCAGGAGGATGGCATGAATGTATGGCTGGCTGACCTTGATCCCTTTTGTGCATCTCTTATGCCCTCCACAG
Seq C2 exon
TGGAGGGCATCGTGGGGCTGGAGCAAGTAAGCAGCCTTGGAGATGGCCCACCGGGAGGCCGCCCCCGCCGTGGAATGTTCCGGACTGTGGGGCAGCTCTACAAAGAATCCCTGAGCCGCCTCATGGCCACGCTCAGCAACACCAACCCTAGTTTTGTCCGCTGCATCGTTCCCAATCATGAGAAGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030739-Myh14:NM_028021:14
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.019 A=NA C2=0.258
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0006316=Myosin_head=FE(5.5=100)

							
A:
NA

							
C2:
PF0006316=Myosin_head=FE(9.0=100)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000046059





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000130682
          









    
Other Skipping Isoforms:
ENSMUSP00000103531, ENSMUSP00000103532
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr19:50252754-50255219 
ALTERNATIVE
HsaINT0107588
(MYH14)
Human
(hg19)
chr19:50756011-50760555 
LOW PSI
HsaINT0107588
(MYH14)
Rat
(rn6)
chr1:100635120-100638396 
ALTERNATIVE
RnoINT0096389
(Myh14)
Cow
(bosTau6)
chr18:56909617-56911191 
LOW PSI
BtaINT0097185
(MYH14)
Chicken
(galGal4)
chr18:1724611-1728147 
LOW PSI
GgaINT0127245
(MYH10)
Chicken
(galGal3)
chr18:1714138-1715174 
LOW PSI
GgaINT0127245
(Q789A6_CHICK)
Zebrafish
(danRer10)
chr6:7457451-7466790 
LOW PSI
DreINT0099725
(myh10)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
No suggested primer sequences

				
R: 
No suggested primer sequences

				
Band lengths: 

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"