BtaINT0097272 @ bosTau6
Intron Retention
Gene
ENSBTAG00000007090 | MYH2
Description
Bos taurus myosin, heavy chain 2, skeletal muscle, adult (MYH2), mRNA. [Source:RefSeq mRNA;Acc:NM_001166227]
Coordinates
chr19:30145076-30146691:-
Coord C1 exon
chr19:30146302-30146691
Coord A exon
chr19:30145203-30146301
Coord C2 exon
chr19:30145076-30145202
Length
1099 bp
Sequences
Splice sites
5' ss Seq
AAGGTACTG
5' ss Score
8.56
3' ss Seq
TTCCACTTTTCCTCTCACAGGGA
3' ss Score
10.63
Exon sequences
Seq C1 exon
GCCCGCATCGAGGAGCTGGAGGAGGAGATCGAGGCCGAGCGGGCCTCCCGCGCCAAGGCAGAGAAGCAGCGCTCAGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGCGGCTAGAAGAAGCCGGCGGGGCCACTTCCGCCCAGATTGAGATGAACAAGAAGCGGGAGGCCGAGTTCCAGAAGATGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGCGGCCGCTCTTCGCAAGAAGCACGCGGACAGTGTGGCTGAGCTGGGGGAGCAGATTGACAACCTGCAGAGGGTCAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGACGACCTTGCTAGCAATGTGGAAACGATCTCTAAAGCCAAG
Seq A exon
GTACTGCCGAGTCTGAGAAATTGTTATTAACAGCAATGACCCATTATGTGCACAGTCTGCATAGGGCCTTTACACACAGGTGATTTGTTCTTTGCAGTAATCCAGGAGGTAAACAAGAAAGATACCAGTATTATATTATTTCCACTCAATCATAGGAAGAAACTAAAATTCAAAGAAGCTGAGCATATTAACCCCACCACACTATTAACATAGGACAACATCTGCAGCGAACCAGGCTGTCCGTGTTCTCTAGCACTTCAACTGGGGAACACAGGTCTCCAAAATTCTCAACTATCCAACATGCAGTTTCACTGTACATAAGTAGGCAGTGAAGTATTTATAAATGCTTTTTGAAAATATGTCACTACATTTTTCACAAAATCTTTAGTACATGATCTTTCATTTTTTTATCACAGGCAGACCTCAGAGATATTGCAGGTTTGGTTCCAGACCACCACAATGAAGCAAATATCAAAATAAAGCAAGCCACTCAAATTCCCTGGTTTCCCAAGGCGTATAAAAATGATGTTTACACTATAATGTAGTCTATTAAGTGTGCAACAGCATTATGCCTAAAAAAAAATGCATCCCTTCATTTAAAAATACTTTCTTGCTTAAAAAAAAAAAATGCTAACCATCATCTGAACCATCAATGAGTCACAGTGCCAACATCAAAGATCACAGATCACTGAAACAAATATAATAATGAAGAAAAGCTTGAATACTGTGAGAATTACCAAAATGTGATACAGAGACATGAAGTGGGCCAATGCTATTGGAAAAATAGTGCTGATAGACTTGCTGGATACAGGGTTGCCCAAACCCTAAATTTGTTTTTAAAAAAAAGTGATATCTGGGAAGAGCGATAATACAAGGAATGCCTGGAATAATGAATGAAGCTTCACAATTAGCTTTTTAAAACTCCCAATGTGTGTCTTCACCTTAGGACAGCAATCATATTATACAAGATAAAGAAAAAATTCCTATATATTACATACAAGATAATTTACTTTATATGTCAAAATAAATCTTTTTCTATTTTAGAAGAAATGGTAGCTGTCAATGTCTGCACTTGCTTCTTCCACTTTTCCTCTCACAG
Seq C2 exon
GGAAACCTAGAGAAGATGTGCCGAACTCTGGAGGACCAAGTGAACGAGCTGAAATCCAAGGAGGAGGAGCAGCAGCGGCTGATCAATGACCTGACAACCCAGAGGGGACGCTTGCAGACTGAATCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000007090:ENSBTAT00000012797:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.892 A=NA C2=0.953
Domain overlap (PFAM):
C1:
PF0003816=Filament=FE(45.4=100),PF0157614=Myosin_tail_1=FE(15.0=100)
A:
NA
C2:
PF0003816=Filament=FE(14.8=100),PF0157614=Myosin_tail_1=FE(4.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAACTGGAGGAGATCAGCGA
R:
CAGCTCGTTCACTTGGTCCTC
Band lengths:
355-1454
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]