BtaINT0103665 @ bosTau6
Intron Retention
Gene
ENSBTAG00000019327 | NRAP
Description
nebulin-related anchoring protein [Source:HGNC Symbol;Acc:HGNC:7988]
Coordinates
chr26:34341289-34343471:-
Coord C1 exon
chr26:34343364-34343471
Coord A exon
chr26:34341601-34343363
Coord C2 exon
chr26:34341289-34341600
Length
1763 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGA
5' ss Score
5.46
3' ss Seq
TCTGCTCCTCTGCCCTGCAGTTC
3' ss Score
9.21
Exon sequences
Seq C1 exon
AAAGTCTACAGACACTCCTGGGAGCAGACCCGGGCTGGCGGCTACGACTTCAGGCTGGATGCCATCCCATTCCAGACGGCACGGGCATCGAGGGAAATCGCCAGTGAT
Seq A exon
GTAAGACCTCACGTTCCCTCCCTGCCTCTGTCTCTGATAGGTGTCACTCTGGTTTCCCCAGTTCTGTTGAAAGGCAGACCACTGGGAACCAAAGCTGAGAAAGTATTTCAAGTGAAAGGAATAAAAGTTGGCCTCAATTTCTAGCATCTCAGCCAGAAATTTGGAATTAGGGAAGGTTTTATTCCTATTTCCTCTCCTTCAAGAGAGGCGTTCTTAAATGTTGATGCATGTGGACAGAAACTAGAAATCAGTTAGCAGAGGACTGATTTGTTGACCTTCAATAAAGGGACCTTTTTTTTCAAAGCTGAAGTGCACTATGGAATAGAAAGAGATTGGGTTTCCCGCCTAGATTGACTTGAGTTCAGATCCTGCCTCAGTTGCTTAGGAGTTGGCTCTGTGGTCTTGAGCAAGTTGCTTAAATCCTCTGAACTAAAATTTCTCCTATGAAATAGAGATCTAATATTTGTCTCTATTGGTGACAGGATTGATGTAGAGAGAGTGAAAGTTGCTCAGTCGTATCCAACTCTTTGCCACTCCATGAACTATACAGTCCATGGAATTCTCCAGGCCAGAATACTGGAGTGGATAGCTTTTCCCTTTTCCAGGGGTCTTCCCAACCCAGGGATAGAACCGCGGTCTCATGCATTGCAGGCGGATTCTCTACCAGCTGAGCCAGCAGGGAAGCCCGGGATTGATGTAGGAATGTAACCAAAATGGTTCAGTGTCATGTACAGAGCAAGGGGACAAAGCATGGCAGCTGTTATTATCGAAGAGGAAGACATCACTAACTAGATAGGAAGACATTACTAACTAGATATATGATCATTCATGAACAACTTGTCACTGGTTCAAAGTGAAACAAGTATAGAAACCAAGCATTTAGCAACACTTAGCAATGAGATATTCTGGCCTCCAAGCCTGAGATGGGTGGACTCCTCTGGCTGAGCAGAATAGAGGCCAGTTTGGGTTTATCAAATGCACCTGGTACTTTTTACATACTAATCACAGGCTTCCCAGGTGGCGCTAGCGGTAAAGAACCCGAGTGCCAATGCAGGAGACATAAGAGATGCGGTTTGATCCCTGGGTGGGGAAGATCCTCTGGAGGAGGGCACGGCAACCCACTCCAGTATTTTTGCCTGGGGAATCCCATGGACAGAGGAGCCTGGCGGCTACAGTCCATGGGGTCACACAGAGTCAGACACGACTGAAGTGATTTCACACACATGCAATCACAGGCAGAAGGACTACATATTGGACTTCGATGGATTGGAAATTTTAAAAAAGAAAGTGACTCAGTCCCGTTTTTCACTATATATGGTTTGAGGAGCACTGAGTTGGGTCCCTGAAAGCTGGAAAGAATCAAAGGATGAGTGGCTGCTGATAGAATGGGTGCACTCCTTAACTGTTGGATTAACTGTTACCACAGCAATACCGTCACCTGCCGCTGGGTGGACGTGAGCCTGTGCAGGACATGAAGCTCAGCCCCTCTCGCTGAATCCTCTCAAGATCCCGTGGGGTGGAGGTTCCCGCACCCATTTTCACACCCATTTTACCAAGTGGGGAGACCAAAGCTCAGAGAGACTCAGTAACTGACCCGAGGCCACACAGCTAAAGGAAACAGAGGGAAACCCGGATCCCAGGCCTACTTGAATCCAAAGCGGTGCCCTTGACCCACTGCTGAACTTCCTGCTGAGAATCCCGAGGGCCGGCCCTGGAGGGAAGGGGGGCGCCCATGACCACCTCTCTGCTCCTCTGCCCTGCAG
Seq C2 exon
TTCCGGTACAAAGAGGCCTTCCTACGGGACCGGGGCCTGCAGATAGGGTACCGCAGCATCAACGACGACCCGAGGACGAAGCATTTCCTCAGCGTCGGCAGACTCCAGAGTGACAACGAGTACAAGAAGGACTTCGCCAAGAGTCGGTCCCAGTTCCACAGCCGCCCAGACCAGCCCGGCTTCCTCCAGGCCAAGAGGAGCCAGCAGCTGGCCAGCGACGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTGAAACACGCGCAGAAGGCCCACCGGCTGCAGAGCGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000019327:ENSBTAT00000025740:39
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.654
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=10.6),PF0088013=Nebulin=WD(100=27.9),PF0088013=Nebulin=WD(100=21.2),PF0088013=Nebulin=PU(53.6=14.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGCCATCCCATTCCAGACG
R:
CCTTCTGCGCGTGTTTCAGG
Band lengths:
343-2106
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]