RnoINT0103062 @ rn6
Intron Retention
Gene
ENSRNOG00000016714 | Nrap
Description
nebulin-related anchoring protein [Source:RGD Symbol;Acc:1306731]
Coordinates
chr1:277106164-277107706:-
Coord C1 exon
chr1:277107599-277107706
Coord A exon
chr1:277106476-277107598
Coord C2 exon
chr1:277106164-277106475
Length
1123 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGA
5' ss Score
5.46
3' ss Seq
TCTGCTTCTCTGACCTGCAGTTC
3' ss Score
8.89
Exon sequences
Seq C1 exon
AAAGTCTACAGAAATTCCTGGGAGAAGAACCGGGCTGGCGGCTATGACTTCAGGCTGGATGCTATCCCGTTCCAGACTGCACGGGCATCCAGGGATATTGCCAGTGAT
Seq A exon
GTAAGACTCCCAATCCTGCCTGCAGATGATAAAAGCTCTCCCTAGTTATCCAAGTGTGCCGAAAGGCACAGGGCAGACTCTCGGGCCACCAGATTGACTTTCTCCACACTAGAATGGAAACAATGTATATTTCAAAGGGGCGAGAACAAGGGCCTGGCTGTTAGGATTGTAGACAGAAGTATGAGGTTGGGAAGGGTTTGTTTTGGTTTGGTTTTTTGGCAACTTTTTTTGGGAGAGGTACACAGCAGTTGGTGTGGCACACCAGGACGCCAATGAACCGGTGCTTCTGTGTCTGCTTAATCTGTAGCAACAAACCTCTTGTGAAGTTGCTTCTGTGCAAGCATGGGGACTTATGTTCAGACTCATAGATCATAGGTCATAAAGTCAGATGCGGTGGTTCGTGTCGGTAGCCCCAGATGTGATCGCTTGGATATGCACTAGGTAACGTCAGACTTTCTAACACTGCAGAAGGCCAAGAGTTGTCCTCTGTCCTCGACAGGTACATGGTGGCACATGTCCCTCAACTCACACACTCGAATTGATACATGTACACATACACACAAGATAAAATAAGAAGCGTGACTGTGTAAAATGAACTAGATTCTTCGCTTTGAGCAATTTGAGAAGCACTACCTTTGGTCCCTGAATGTGGGGAGGTCAGAAGAAAGAATGACTCACTTCTATTCCTGTTGGAGTGGCACTTACAGCAGCCATGCTGGGTTACAGGTGCATCCTTCCACACCTGAGGTTACAGGAGCACCCTAGCACATCCTATGTTTCAGCTATATACTGTTATATCTGAGGTTACAGGTATACACTACCACATTCTAGGTTACAGGTATACATAAAGGCGTCATCAGTAGGTGTGGATAACATTTCAGGCCCATATCATTGACCTCTCTCAACATTCGTGAGGTGGAAGTTTGCTCTCCTGTTTGATCAGATGTTCAGAGAGGCTACATAGCTGTTCCAAGGCAGTACAGCTTAGAGCTGATTCCAAAGCTTATGCCCTTATCCACTGTTGAGCTTCCTGCTGAAGTCCTGGGGTCTGTCCTCTCTAGGGAGGCTGGCTTTGAAGGGAACAGACACCCCCCTCATCTCTCTCTGCTTCTCTGACCTGCAG
Seq C2 exon
TTCCGGTACAAAGAGGCATTTCTGAGGGACCGGGGCCTACAGATTGGGTATCGCAGCGTCAATGACGACCCAAGGATGAGACATTTCCTCCGAGTCGGCAGGCTCCAAAGTGACAATGAGTACAGAAAGGACTTCGCCAAAGGTCGCTCCCAGTTCCACAGTCGTCCTGACCAACCTGGCTTCCTCCAGGCCAAGAGGAGCCAGCAACTGGCCAGTGATGTGCTCTACAGGCAGCCACTGCCCCAGCACACCAGCGACCCAGAAGAGCTGGGCCTGAAGCACGCCCGGAAGGCTCATCAGCTACAGAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016714:ENSRNOT00000038017:38
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.548
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=10.6),PF0088013=Nebulin=WD(100=27.9),PF0088013=Nebulin=WD(100=21.2),PF0088013=Nebulin=PU(51.9=13.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGCTATGACTTCAGGCTGGA
R:
CTTCAGGCCCAGCTCTTCTG
Band lengths:
349-1472
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]