BtaINT0120955 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018928 | QARS
Description
glutamine--tRNA ligase [Source:RefSeq peptide;Acc:NP_001029640]
Coordinates
chr22:51484527-51485137:+
Coord C1 exon
chr22:51484527-51484674
Coord A exon
chr22:51484675-51485027
Coord C2 exon
chr22:51485028-51485137
Length
353 bp
Sequences
Splice sites
5' ss Seq
GCGGTGAGA
5' ss Score
7.4
3' ss Seq
ATCTGTTCGCTCTTTTTCAGCTG
3' ss Score
9.82
Exon sequences
Seq C1 exon
GCGCAGCAGACTCTGGGCTCCTCCATCGACAAGGCTACCGGGACCCTGCTCTATGGATTGGCCTCCCGACTCCGGGATCCTCGGCGCCTTTCTTTCCTCGTGAGCTACATAACCAGTAGGAAGATCCACACTGAGACCCAGCTGAGCG
Seq A exon
GTGAGACCCCTCCCTGACCTGCGCTTCCACCTCAAACTTCTCTCAGCCCAGACCCCTTTCTGTCCCTTCTGAGAAGTCCCTCGGTGTCTCTCTAGATTACTAGTTCTCTAAACCGAGGCCCTGGCACTGACAGATGGAGAGGGCCACCTCTGGTGAGACTGACTTAGAGAAACCTAGGTCTGATGAGGAATTTCCCTCAGAATACATGCTTCTTTGCTGAGGCCTCAGAGCTTCTCTAGAGGGGCACGACTTAATTTCCCAGGAGCCAAATTTATTGGGGTTTCCTGGATGGATTAGGGTCTTGTCTTATTTCTTGGAGCTGAGATGCCACAAATCTGTTCGCTCTTTTTCAG
Seq C2 exon
CTGCCCTTGAGTATGTGCGGAGTCATCCCTTGGATCCCATCAACACTGAGGACTTCGAGCAGGAATGCGGTGTGGGCGTTGTGGTGACCCCAGAACAGATTGAAGAGGCC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018928:ENSBTAT00000025191:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0455810=tRNA_synt_1c_R1=FE(30.1=100)
A:
NA
C2:
PF0455810=tRNA_synt_1c_R1=FE(22.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGCAGACTCTGGGCTCCTC
R:
GCCTCTTCAATCTGTTCTGGGG
Band lengths:
254-607
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]