MmuINT0130118 @ mm10
Intron Retention
Gene
ENSMUSG00000032604 | Qars
Description
glutaminyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:1915851]
Coordinates
chr9:108508383-108508986:+
Coord C1 exon
chr9:108508383-108508530
Coord A exon
chr9:108508531-108508876
Coord C2 exon
chr9:108508877-108508986
Length
346 bp
Sequences
Splice sites
5' ss Seq
GCGGTGAGT
5' ss Score
10.49
3' ss Seq
AATTGCCCCTCCGTTTTCAGCTG
3' ss Score
10.6
Exon sequences
Seq C1 exon
GCACACCAGATTCTGGGTTCTACCATCGACAAGGCTACTGGGGTCCTCCTATATGACTTGGTCTCCCGACTCAGGGATACTCGGCGTCGTTCTTTCCTTGTGAGCTATATAGCCAATAAGAAGATTCACACGGGACTCCAGCTGAGCG
Seq A exon
GTGAGTCCCGTCTGTTTTACTAGTCCACCTTCAATCTCTTCTTTAGCAAAGACTCGAGTTTGCTTTTTCTGTCAAGTGTTGCTTTTTGGACAGCAGGTTCTGGACAGGCACTGAGGCATGGAGGTAGAGACCTCTGGTGTGGATTGCAGAGTTACCACAATGAGAAGCCTGCTTCAGAACACATGGCTTCTCTTTTCTCTGGTCTCAGTGCTTCTCTTCAGTGGTGCCAGGGCCTAACCTCTTAGGCATCTAAGTAACCGAAATAGTTACCTCTTCACTGTACTAAGGCCCTATCTACCCACTTCTTGGAGCTGACTTGTCTAGTTAATTGCCCCTCCGTTTTCAG
Seq C2 exon
CTGCTCTTGAATATGTTCGGAGTCATCCCCAGGATCCCATTGATACCAAGGACTTCGAGCAGGAGTGTGGCGTCGGTGTGGTGGTGACACCGGAGCAGATTGAGGAAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032604:ENSMUST00000006838:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.065
Domain overlap (PFAM):
C1:
PF0455810=tRNA_synt_1c_R1=FE(41.6=100),PF116623=DUF3263=PD(54.4=93.9)
A:
NA
C2:
PF0455810=tRNA_synt_1c_R1=FE(22.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGGTTCTACCATCGACAAGG
R:
AGCTTCCTCAATCTGCTCCGG
Band lengths:
246-592
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types