BtaINT0120958 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018928 | QARS
Description
glutamine--tRNA ligase [Source:RefSeq peptide;Acc:NP_001029640]
Coordinates
chr22:51490841-51491107:+
Coord C1 exon
chr22:51490841-51490907
Coord A exon
chr22:51490908-51490981
Coord C2 exon
chr22:51490982-51491107
Length
74 bp
Sequences
Splice sites
5' ss Seq
CCGGTAGGC
5' ss Score
7.85
3' ss Seq
CCTGAGTTCTCTGCCCACAGGCC
3' ss Score
9.04
Exon sequences
Seq C1 exon
ATTTCAGCACAAGAACCCCGAGGATCCTGCTGAGGTGCCTGGTGGATTCTTAAGTGACCTGAACCCG
Seq A exon
GTAGGCTCATGGGGTATGGGGTGAGGGTGGTGGGCCTTTCTGGCTCGATAGCCACCTGAGTTCTCTGCCCACAG
Seq C2 exon
GCCTCGCTACAAGTGGTGGAGGCTGCATTAGTGGACTGTTCTGTGGCCCTGGCAAAGCCCTTCGACAAGTTCCAATTTGAGCGCCTTGGTTACTTTTCCGTGGATCCAGACAGCAACCAGGGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018928:ENSBTAT00000025191:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.217 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0395013=tRNA-synt_1c_C=FE(11.8=100)
A:
NA
C2:
PF0395013=tRNA-synt_1c_C=PD(18.2=81.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAAGAACCCCGAGGATCCTG
R:
CTGGTTGCTGTCTGGATCCAC
Band lengths:
179-253
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]